CAJ | 학술논문

目的：建立高效液相色谱法测定万古霉素的血药浓度。方法血浆样品经硫酸锌沉淀处理，采用反相高效液相色谱法，流动相为乙腈-0.02moL?L-1磷酸二氢钾缓冲液（8∶92，V/V），检测波长为236nm，流速为1.0 mL?min-1，柱温为25℃，进样量为10μL。采用峰面积外标法定量测定人血浆中的万古霉素浓度。结果万古霉素进样浓度在2.5～200 mg?L-1范围内与峰面积积分值呈良好的线性关系（r=0.9997，n=7）；日内RSD为3.91%，2.38%，1.77%，日间RSD为4.01%，2.35%，1.82%；相对回收率分别为99.2%～100.3%；稳定性考察结果表明血浆样品在24h内稳定。结论本方法专属性强，简单方便，适用于万古霉素的血药浓度监测。
목적：건립고효액상색보법측정만고매소적혈약농도。방법혈장양품경류산자침정처리，채용반상고효액상색보법，류동상위을정-0.02moL?L-1린산이경갑완충액（8∶92，V/V），검측파장위236nm，류속위1.0 mL?min-1，주온위25℃，진양량위10μL。채용봉면적외표법정량측정인혈장중적만고매소농도。결과만고매소진양농도재2.5～200 mg?L-1범위내여봉면적적분치정량호적선성관계（r=0.9997，n=7）；일내RSD위3.91%，2.38%，1.77%，일간RSD위4.01%，2.35%，1.82%；상대회수솔분별위99.2%～100.3%；은정성고찰결과표명혈장양품재24h내은정。결론본방법전속성강，간단방편，괄용우만고매소적혈약농도감측。
Objective To establish an HPLC method for determination of plasma vancomycin.Methods The vancomycin in plasma extracted by the sedimentation with zinc sulfate was determined by an HPLC method with mobile phase consisted of a mixture of acetonitrile - potassium dihydrogen. Phosphate buffer solution (8∶92, V/V) at the flow rate of 1.0 mL?min-1. The detective wavelength was set at 236nm and the column temperature was 25℃. The injection volume was 10μL.Results The linear range of vancomycin was 2.5~200 mg?L-1(r=0.9997, n=7)with within-day RSD of 3.91%, 2.38%, 1.77% and between-day RSD of 4.01%, 2.35%, 1.82%, the relevant recovery of 99.2%~100.3%.Stability investigation showed that the plasma sample with vancomycin were stable in 24h.Conclusion The method is specific and simple, which can be used for the determination of plasma vancomycin.