山东医药
山東醫藥
산동의약
SHANDONG MEDICAL JOURNAL
2014年
35期
13-15
,共3页
杨文杰%向云涛%程建辉%张向红%马繁荣%王爱红
楊文傑%嚮雲濤%程建輝%張嚮紅%馬繁榮%王愛紅
양문걸%향운도%정건휘%장향홍%마번영%왕애홍
乳浆大戟提取物%肺肿瘤%A549细胞%细胞凋亡
乳漿大戟提取物%肺腫瘤%A549細胞%細胞凋亡
유장대극제취물%폐종류%A549세포%세포조망
extracts of Euphorbia esula%lung tumor%A549 cell%cell apoptosis
目的:观察乳浆大戟提取物对体外培养的人肺癌细胞A549增殖抑制和诱导凋亡的作用。方法将0、0.2、0.4、0.8 g/L的乳浆大戟提取物作用于体外培养的人肺癌细胞A549,采用MTT法观察乳浆大戟提取物对A549细胞增殖的影响;PI染色法观察凋亡细胞的形态;流式细胞仪检测A549细胞的凋亡率。结果乳浆大戟提取物对体外培养的A549细胞增殖有抑制作用,并具有浓度依赖关系。0、0.2、0.4、0.8 g/L乳浆大戟提取物作用A549细胞48 h的凋亡率分别是5.33%±0.67%、15.53%±0.15%、21.42%±1.01%、46.57%±1.76%,三者间两两比较,P均<0.05。结论乳浆大戟提取物可通过诱导A549细胞凋亡而抑制细胞增殖。
目的:觀察乳漿大戟提取物對體外培養的人肺癌細胞A549增殖抑製和誘導凋亡的作用。方法將0、0.2、0.4、0.8 g/L的乳漿大戟提取物作用于體外培養的人肺癌細胞A549,採用MTT法觀察乳漿大戟提取物對A549細胞增殖的影響;PI染色法觀察凋亡細胞的形態;流式細胞儀檢測A549細胞的凋亡率。結果乳漿大戟提取物對體外培養的A549細胞增殖有抑製作用,併具有濃度依賴關繫。0、0.2、0.4、0.8 g/L乳漿大戟提取物作用A549細胞48 h的凋亡率分彆是5.33%±0.67%、15.53%±0.15%、21.42%±1.01%、46.57%±1.76%,三者間兩兩比較,P均<0.05。結論乳漿大戟提取物可通過誘導A549細胞凋亡而抑製細胞增殖。
목적:관찰유장대극제취물대체외배양적인폐암세포A549증식억제화유도조망적작용。방법장0、0.2、0.4、0.8 g/L적유장대극제취물작용우체외배양적인폐암세포A549,채용MTT법관찰유장대극제취물대A549세포증식적영향;PI염색법관찰조망세포적형태;류식세포의검측A549세포적조망솔。결과유장대극제취물대체외배양적A549세포증식유억제작용,병구유농도의뢰관계。0、0.2、0.4、0.8 g/L유장대극제취물작용A549세포48 h적조망솔분별시5.33%±0.67%、15.53%±0.15%、21.42%±1.01%、46.57%±1.76%,삼자간량량비교,P균<0.05。결론유장대극제취물가통과유도A549세포조망이억제세포증식。
Objective To investigate the inhibit proliferation and induced apotosis effects of extracts of Euphorbia esu-la in the human lung cancer cell lines A549.Methods The human lung cancer cell lines A549 were treated with different concentrations(0, 0.2, 0.4, 0.8 g/L) of extracts of Euphorbia esula in vitro, the cell proliferation was analyzed by MTT;the cell morphological changes were observed using PI dyeing;the cell apoptosis was detected by flow cytometry.Results the growth of A549 cells were inhibited by extracts of Euphorbia esula in a dose-dependent manner.A549 cells were treated with extracts of Euphorbia esula of 0, 0.2, 0.4, 0.8 g/L concentrations for 48 h, the apoptosis rates were 5.33%±0.67%, 15.53%±0.15%, 21.42%±1.01%, 46.57%±1.76%, respectively.Compared with the former, the lat-ter was higher, all P<0.05.Conclusion Extracts of Euphorbia esula can inhibit apoptosis of the A549 cells by inhibition of cell proliferation.