CAJ | 학술논문

大豆球蛋白是豆粕饲料的主要营养成分之一，对豆粕饲料的营养品质有着重要影响，其中7S伴大豆球蛋白及11S球蛋白是大豆球蛋白最重要的成分。研究分析了在不同提取液，不同的pH，不同的温度，不同浓度的Tris-HCl提取液及不同料液比对豆粕大豆球蛋白提取的影响，并且对7S伴大豆球蛋白和11S球蛋白进行了分析。最终SDS-PAGE结果显示55℃、0．05 mol/L、pH9．0的Tris-HCl溶液在料液比为1∶10时能够提取较多的球蛋白，并且7S伴大豆球蛋白由3个亚基组成，其相对分子量分别为60466 Da，55029 Da和49043 Da，11S球蛋白也由3个亚基组成，其相对分子量分别为44634 Da，39779 Da和17952 Da。
대두구단백시두박사료적주요영양성분지일，대두박사료적영양품질유착중요영향，기중7S반대두구단백급11S구단백시대두구단백최중요적성분。연구분석료재불동제취액，불동적pH，불동적온도，불동농도적Tris-HCl제취액급불동료액비대두박대두구단백제취적영향，병차대7S반대두구단백화11S구단백진행료분석。최종SDS-PAGE결과현시55℃、0．05 mol/L、pH9．0적Tris-HCl용액재료액비위1∶10시능구제취교다적구단백，병차7S반대두구단백유3개아기조성，기상대분자량분별위60466 Da，55029 Da화49043 Da，11S구단백야유3개아기조성，기상대분자량분별위44634 Da，39779 Da화17952 Da。
Soybean glycinin,which is one of the major compositions in soybean meal,greatly influences the quality of soybean meal,and the most important components of glycinin are 7s-conglycinin globulins and 11S globulins.The objective of the study was to decide the optimal conditions for extraction of soybean protein and to analyze 7s-conglycinin globulins and 11S globulins.The results of SDS-PAGE showed that 0.05 M Tris-HCl ( pH9.0,55 ℃) was the optimal condition for the extraction of soybean glycinin with the sample-solution ratio 1 ∶10, and 7s-conglycinin globulins were composed of three subunits with molecular weight of 60466Da, 55029Da and 49043Da,respectively and 11S globulins were also composed of three subunits with molecular weight of 44634Da,39779Da and 17952Da,respectively.