CAJ | 학술논문

以基因疫苗pVGS/2SS-asd为模板，采用分子生物学方法，分别获得目的片段粒细胞-巨噬细胞集落刺激因子（ GM-CSF）和含有乙肝表面抗原S基因的生长抑素基因（ SS）。以共表达质粒pIRES为载体，采用基因工程方法，将GM-CSF和SS基因片段分别克隆到载体的多克隆位点A和多克隆位点B上，构建共表达基因疫苗pIRES-GM-CSF/2SS，旨在同时表达GM-CSF和SS两种蛋白。获得表达质粒后，酶切和测序鉴定该共表达基因疫苗，结果显示，该共表达基因疫苗pIRES-GM-CSF/2SS构建成功，为该共表达基因疫苗的后续研究奠定了良好的基础。
이기인역묘pVGS/2SS-asd위모판，채용분자생물학방법，분별획득목적편단립세포-거서세포집락자격인자（ GM-CSF）화함유을간표면항원S기인적생장억소기인（ SS）。이공표체질립pIRES위재체，채용기인공정방법，장GM-CSF화SS기인편단분별극륭도재체적다극륭위점A화다극륭위점B상，구건공표체기인역묘pIRES-GM-CSF/2SS，지재동시표체GM-CSF화SS량충단백。획득표체질립후，매절화측서감정해공표체기인역묘，결과현시，해공표체기인역묘pIRES-GM-CSF/2SS구건성공，위해공표체기인역묘적후속연구전정료량호적기출。
With gene vaccine pVGS/2SS-asd for template,target fragments of granulocyte-macrophage colony-stimulating factors ( GM-CSF) and somatostatin ( SS) gene contained the S gene of HBsAg were ob-tained by molecular biology.With co-expression plasmid pIRES for vector, GM-CSF and SS gene fragments were cloned to MCSA( multiple cloning site A) and MCS B ( multiple cloning site B) of pIRES by genetic engi-neering.Co-expression gene vaccine of GM-CSF and SS ( pIRES-GM-CSF/2SS) was constructed.It was de-signed to expression protein of GM-CSF and protein of SS gene simultaneously.pIRES-GM-CSF/2SS was i-dentified with enzyme digestion and sequencing.The results showed that co-expression gene vaccine pIRES-GM-CSF/2SS was successfully constructed,which provides a material foundation for immunomodulation of GM-CSF on DNA vaccine.