CAJ | 학술논문

为摸清大鳞鲃在碱性水域推广的可行性，对大鳞鲃（ Barbus capito）、草鱼（ Ctenopharyngodon idllus）、镜鲤（ Songpu mirror carp）、鲫鱼（ Carassius auratus）和白鲢（ Hypophthalmichthys molitrix）幼鱼进行96 h急性NaHCO3碱度毒性试验，测定了水体中NaHCO3碱度对5种鱼的致死浓度，以及对鳃、肾组织的影响。结果显示，大鳞鲃、草鱼、镜鲤、鲫鱼和白鲢5种幼鱼NaHCO3碱度96 h半致死浓度LC50分别为123．325 mmol/L，91．825 mmol/L，114．262 mmol/L，134．045 mmol/L，86．246 mmol/L，其耐受能力大小顺序依次为：鲫鱼，大鳞鲃，镜鲤，草鱼，白鲢。观察了NaHCO3碱度对鳃和肾脏的病理组织切片，在高碱胁迫下，鳃小片缩短，细胞间隙增大，鳃小片上皮细胞出现不同程度的水肿，甚至脱落；肾组织中肾小管出现不同程度的萎缩，肾小管管腔变大。
위모청대린파재감성수역추엄적가행성，대대린파（ Barbus capito）、초어（ Ctenopharyngodon idllus）、경리（ Songpu mirror carp）、즉어（ Carassius auratus）화백련（ Hypophthalmichthys molitrix）유어진행96 h급성NaHCO3감도독성시험，측정료수체중NaHCO3감도대5충어적치사농도，이급대새、신조직적영향。결과현시，대린파、초어、경리、즉어화백련5충유어NaHCO3감도96 h반치사농도LC50분별위123．325 mmol/L，91．825 mmol/L，114．262 mmol/L，134．045 mmol/L，86．246 mmol/L，기내수능력대소순서의차위：즉어，대린파，경리，초어，백련。관찰료NaHCO3감도대새화신장적병리조직절편，재고감협박하，새소편축단，세포간극증대，새소편상피세포출현불동정도적수종，심지탈락；신조직중신소관출현불동정도적위축，신소관관강변대。
In order to verify the promoted feasibility of Barbus capito in alkaline waters,experiments were conducted to explore the half lethal concentrateion ( LC50 ) of NaHCO3 in juveniles of 5 species through 96 h a-cute alkalinity toxicity test with fresh water test for comparison,and its the impact on gill,kidney tissues was ob-cerved.According to the results of the acute alkalinity experiment,the LC50 of alkalinity in Barbus capito,Ctenopha-ryngodon idllus, Songpu mirror carp, Carassius auratus, and Hypophthalmichthys molitrixwere 123.325 mmol/L, 91.825 mmol/L,114.262 mmol/L,134.045 mmol/L,86.246 mmol/L,respectively.Therefore,the sequence of alkali-tolerance was:Carassius auratus>Barbus capito>Songpu mirror carp>Ctenopharyngodon idllus>Hypoph-thalmichthys molitrix.Furthermore,the gill and kidney structures of 5 juveniles were observed under NaHCO3 by using the technology of histological photomicrography.The results showed that gill lamellae narrowed,inter-cellular space increased and edema appeared in the epithelial cells in different degrees,the cells or even fell off;atrophy appeared in renal tubules in different degrees.