江西农业大学学报
江西農業大學學報
강서농업대학학보
ACTA AGRICULTURAE UNIVERSITATIS JIANGXIENSIS
2014年
5期
1109-1114
,共6页
西伯利亚鲟%流速%行为指标%抗氧化酶%盐酸恩诺沙星残留
西伯利亞鱘%流速%行為指標%抗氧化酶%鹽痠恩諾沙星殘留
서백리아심%류속%행위지표%항양화매%염산은낙사성잔류
Siberian Sturgeon%water velocity%behavior index%antioxidant enzyme%residues of enrofloxacin-HCl
以西伯利亚鲟幼鱼[体质量（82．1±4．9）g]为实验材料，设置0 BL/s对照组，1．0和2．0 BL/s （体长/秒）3种流速条件，于处理后1 d，15 d和30 d 取样，进行鱼类行为学指标和抗氧化酶的测定。在此基础上，以50 mg/kg剂量，连续7 d混饲给药，研究了流速对停药后西伯利亚鲟肌肉组织中盐酸恩诺沙星残留的影响。结果表明：和静水对照组相比，实验期间各时段2个流速组西伯利亚鲟摆尾频率和趋流率均显著上升（ P＜0．05）。1．0 BL/s组流速下有最高的趋流率，2．0 BL/s组流速下有最高的摆尾频率；同一流速下西伯利亚鲟摆尾频率和趋流率随养殖时间的延长没有明显差异。 CAT、SOD和MDA 活力随着流速的增加而显著增强，两流速组均显著大于静水组（ P＜0．05），2．0 BL/s组流速下活力最高；3种抗氧化酶活力随养殖时间的延长差异均不显著。与静水对照组相比，停药后各时间点2．0 BL/s流速组鱼肌肉盐酸恩诺沙星含量均显著减少（ P＜0．05），在停药第8天已低于检出限，1．0 BL/s流速组鱼在停药第12天已低于检出限，而静水对照组则在停药20 d才低于检出限。研究结果说明适当的水流刺激可以诱导鱼类进行逆流运动，促进鱼体抗氧化酶活力的激活和加速药物残留的排除。
以西伯利亞鱘幼魚[體質量（82．1±4．9）g]為實驗材料，設置0 BL/s對照組，1．0和2．0 BL/s （體長/秒）3種流速條件，于處理後1 d，15 d和30 d 取樣，進行魚類行為學指標和抗氧化酶的測定。在此基礎上，以50 mg/kg劑量，連續7 d混飼給藥，研究瞭流速對停藥後西伯利亞鱘肌肉組織中鹽痠恩諾沙星殘留的影響。結果錶明：和靜水對照組相比，實驗期間各時段2箇流速組西伯利亞鱘襬尾頻率和趨流率均顯著上升（ P＜0．05）。1．0 BL/s組流速下有最高的趨流率，2．0 BL/s組流速下有最高的襬尾頻率；同一流速下西伯利亞鱘襬尾頻率和趨流率隨養殖時間的延長沒有明顯差異。 CAT、SOD和MDA 活力隨著流速的增加而顯著增彊，兩流速組均顯著大于靜水組（ P＜0．05），2．0 BL/s組流速下活力最高；3種抗氧化酶活力隨養殖時間的延長差異均不顯著。與靜水對照組相比，停藥後各時間點2．0 BL/s流速組魚肌肉鹽痠恩諾沙星含量均顯著減少（ P＜0．05），在停藥第8天已低于檢齣限，1．0 BL/s流速組魚在停藥第12天已低于檢齣限，而靜水對照組則在停藥20 d纔低于檢齣限。研究結果說明適噹的水流刺激可以誘導魚類進行逆流運動，促進魚體抗氧化酶活力的激活和加速藥物殘留的排除。
이서백리아심유어[체질량（82．1±4．9）g]위실험재료，설치0 BL/s대조조，1．0화2．0 BL/s （체장/초）3충류속조건，우처리후1 d，15 d화30 d 취양，진행어류행위학지표화항양화매적측정。재차기출상，이50 mg/kg제량，련속7 d혼사급약，연구료류속대정약후서백리아심기육조직중염산은낙사성잔류적영향。결과표명：화정수대조조상비，실험기간각시단2개류속조서백리아심파미빈솔화추류솔균현저상승（ P＜0．05）。1．0 BL/s조류속하유최고적추류솔，2．0 BL/s조류속하유최고적파미빈솔；동일류속하서백리아심파미빈솔화추류솔수양식시간적연장몰유명현차이。 CAT、SOD화MDA 활력수착류속적증가이현저증강，량류속조균현저대우정수조（ P＜0．05），2．0 BL/s조류속하활력최고；3충항양화매활력수양식시간적연장차이균불현저。여정수대조조상비，정약후각시간점2．0 BL/s류속조어기육염산은낙사성함량균현저감소（ P＜0．05），재정약제8천이저우검출한，1．0 BL/s류속조어재정약제12천이저우검출한，이정수대조조칙재정약20 d재저우검출한。연구결과설명괄당적수류자격가이유도어류진행역류운동，촉진어체항양화매활력적격활화가속약물잔류적배제。
The influence of water velocity (0,1.0 and 2.0 BL/s),on behavior,antioxidant enzyme and residues of enrofloxacin in Siberian Sturgeon Acipenser baerii[weight (82.1±4.9)g] was studied.The experiments were divided into 1 d,15 d and 30 d in three stages.On this basis experiments were conducted to study the res-idues and depletion of enrofloxacin-HCl of Acipenser baerii after different water velocity treatments ( 0,1.0 and 2.0 BL/s) .Muscle was sampled after oral administration at the dose of 50 mg/kg for seven consecutive days.It was found that the rheotaxis frequency ( RF) and the tail beat frequencies( TBF) showed a similar tendency:the RFand TBF of 1.0 BL/s and 2.0 BL/s groups were significantly higher than those of 0 BL/s group ( P<0.05) ,of which the maximum RF appeared in 1.0 BL/s in most periods of time and the maximum TBF was in 2.0 BL/s.The two indexes changed little with prolongation of time.It was also found that the MDA,CAT and SOD activities showed a similar tendency:increased with the increase in water velocity.The maximum antioxi-dant enzymes all appeared in 2.0 BL/s.The three indexes of 1 BL/s and 2 BL/s groups were significantly high-er than those of 0 BL/s group( P<0.05) .The three indexes remained stable with prolongation of time.In most time the residue levels of enrofloxacin-HCl of 1 BL/s and 2 BL/s groups were significantly lower than those of 0 BL/s.No medicine residues were detected in 8d under 2.0 BL/s,12 d under 1.0 BL/s while 20 d in the con-trol.The results showed that higher water velocity could induce rheotaxis behaviour,activate antioxidant enzyme and speed drug elimination.
