江西农业大学学报
江西農業大學學報
강서농업대학학보
ACTA AGRICULTURAE UNIVERSITATIS JIANGXIENSIS
2014年
5期
1061-1065,1151
,共6页
李诚%王禄%蒋军喜%王园秀%赵尚高%李帮明%余强%涂贵庆
李誠%王祿%蔣軍喜%王園秀%趙尚高%李幫明%餘彊%塗貴慶
리성%왕록%장군희%왕완수%조상고%리방명%여강%도귀경
猕猴桃果实熟腐病%葡萄座腔菌%生物学特性%品种抗病性
獼猴桃果實熟腐病%葡萄座腔菌%生物學特性%品種抗病性
미후도과실숙부병%포도좌강균%생물학특성%품충항병성
fruit ripe rot of kiwifruit%Botryosphaeria dothidea%biological characteristics%disease resistance of kiwifruit culatvars
为掌握猕猴桃果实熟腐病的发生规律和为其综合防治提供理论依据,对该病害的两种主要病原菌葡萄座腔菌( Botryosphaeria dothidea)和拟茎点霉菌( Phomopsis sp.)的生物学特性、寄主范围和猕猴桃品种抗病性进行了鉴定研究,结果表明葡萄座腔菌和拟茎点霉菌均在25℃和pH 7.0的条件下生长最快;适合两者生长的碳氮源种类基本相同;葡萄座腔菌偏好利用马铃薯葡萄糖培养基、胡萝卜培养基和苜蓿煎汁培养基,而拟茎点霉菌偏好利用烟草煎汁培养基。伤口接种试验表明,供试的5科14种植物均能被上述两种病菌感染,都应属于其寄主范围的植物;供试的17个猕猴桃品种,只有3个品种为高抗品种,其余14个为感病品种。
為掌握獼猴桃果實熟腐病的髮生規律和為其綜閤防治提供理論依據,對該病害的兩種主要病原菌葡萄座腔菌( Botryosphaeria dothidea)和擬莖點黴菌( Phomopsis sp.)的生物學特性、寄主範圍和獼猴桃品種抗病性進行瞭鑒定研究,結果錶明葡萄座腔菌和擬莖點黴菌均在25℃和pH 7.0的條件下生長最快;適閤兩者生長的碳氮源種類基本相同;葡萄座腔菌偏好利用馬鈴藷葡萄糖培養基、鬍蘿蔔培養基和苜蓿煎汁培養基,而擬莖點黴菌偏好利用煙草煎汁培養基。傷口接種試驗錶明,供試的5科14種植物均能被上述兩種病菌感染,都應屬于其寄主範圍的植物;供試的17箇獼猴桃品種,隻有3箇品種為高抗品種,其餘14箇為感病品種。
위장악미후도과실숙부병적발생규률화위기종합방치제공이론의거,대해병해적량충주요병원균포도좌강균( Botryosphaeria dothidea)화의경점매균( Phomopsis sp.)적생물학특성、기주범위화미후도품충항병성진행료감정연구,결과표명포도좌강균화의경점매균균재25℃화pH 7.0적조건하생장최쾌;괄합량자생장적탄담원충류기본상동;포도좌강균편호이용마령서포도당배양기、호라복배양기화목숙전즙배양기,이의경점매균편호이용연초전즙배양기。상구접충시험표명,공시적5과14충식물균능피상술량충병균감염,도응속우기기주범위적식물;공시적17개미후도품충,지유3개품충위고항품충,기여14개위감병품충。
In order to know well the disease occurrence regularity of fruit ripe rot of kiwifruit and provide theoretical basis for comprehensively controlling the disease,the biological characteristics and host range of Bot-ryosphaeria dothidea and Phomopsis sp.,the two main pathogens causing fruit ripe rot of kiwifruit,were studied and the resistance of kiwifruit cultivars to the disease was evaluated.The results showed that at 25℃and pH 7.0,the mycelial growth of both B.dothidea and Phomopsis sp.was the fastest.The varieties of carbon source as well as nitrogen source suitable for the two pathogens to grow were almost same.The optimal medium for B.do-thidea to grow was potato dextrose agar ( PDA) ,carrots agar ( CA) and alfalfa extract agar ( AEA) ,whereas the optimal one for Phomopsis sp.was tobacco extract agar ( TEA) .In the experiment of wounded inoculation,the 14 plant species in 5 families for testing could be infected by both of the two pathogens,which indicated that they were all plant species in their host range.For all the 17 tested cultivars of kiwifruit,only three were highly resistant to B.dothidea and Phomopsis sp.,while the other 14 cultivars were susceptible.