CAJ | 학술논문

为了解唑虫酰胺对小菜蛾实验种群的亚致死效应，为小菜蛾的综合防治提供理论依据，采用浸叶法确定了唑虫酰胺对小菜蛾3龄幼虫的亚致死剂量（ LC30和LC50），研究其对小菜蛾的生长发育、繁殖及其体内解毒酶的影响。结果表明，经唑虫酰胺亚致死浓度（ LC30和LC50）处理后，小菜蛾幼虫体质量与蛹质量减轻，幼虫历期和蛹期明显延长，成虫寿命缩短，羽化率、单雌产卵量与卵孵化率亦均低于对照，小菜蛾3龄期幼虫体内的酯酶、谷胱甘肽-s-转移酶和多功能氧化酶活性均显著低于对照。亚致死浓度的唑虫酰胺对小菜蛾的种群增长有一定的抑制作用，对小菜蛾体内的酯酶、谷胱甘肽-s-转移酶和多功能氧化酶均有明显的抑制作用。这一结果可为唑虫酰胺的科学应用以及制定小菜蛾综合防治策略提供理论依据。
위료해서충선알대소채아실험충군적아치사효응，위소채아적종합방치제공이론의거，채용침협법학정료서충선알대소채아3령유충적아치사제량（ LC30화LC50），연구기대소채아적생장발육、번식급기체내해독매적영향。결과표명，경서충선알아치사농도（ LC30화LC50）처리후，소채아유충체질량여용질량감경，유충력기화용기명현연장，성충수명축단，우화솔、단자산란량여란부화솔역균저우대조，소채아3령기유충체내적지매、곡광감태-s-전이매화다공능양화매활성균현저저우대조。아치사농도적서충선알대소채아적충군증장유일정적억제작용，대소채아체내적지매、곡광감태-s-전이매화다공능양화매균유명현적억제작용。저일결과가위서충선알적과학응용이급제정소채아종합방치책략제공이론의거。
In order to provide the theoretical basis for integrated control of Plutella xylostella,the sublethal effects of tolfenpyrad on experimental populations of P.xylostella were studied.The effects of sublethal doses of tolfenpyrad on growth,development and detoxification enzymes of P.xylostella were investigated with the leaf-dip bioassays,which were used to estimate LC30 and LC50 in advance.Compared with the control,the body mass of the larvae and pupa of P.xylostella was decreased,the development durations of larvae and pupa were obvi-ously prolonged,and the life-span of the adult was shortened after treatment at the LC30and LC50 concentrations of tolfenpyrad.The eclosion rate,the number of eggs laid by per female and hatch rate of eggs were lower than those of the control.The activities of esterase,glutathione-S-transferase and mixed function oxidase of the lar-vae with the treatment of tolfenpyrad were significantly lower than those of the control.The results indicated that tolfenpyrad at sublethal concentrations had some inhibitory effects on the population development of P.xylostel-la,and significant inhibitory effects on the activities of esterase,glutathione-S-transferase and mixed function oxidase of the larvae.This study provides positive guidance for scientific application of tolfenpyrad and develo-ping IPM program for P.xylostella.