江西农业大学学报
江西農業大學學報
강서농업대학학보
ACTA AGRICULTURAE UNIVERSITATIS JIANGXIENSIS
2014年
5期
965-970
,共6页
赵利%陈桂信%潘东明%王玉珍%吕恃衡%姜翠翠
趙利%陳桂信%潘東明%王玉珍%呂恃衡%薑翠翠
조리%진계신%반동명%왕옥진%려시형%강취취
木奈(Prunus salicina Lindl.var.cordata J.Y.Zhang et al.)%乙醇酸氧化酶%全长cDNA%表达分析
木奈(Prunus salicina Lindl.var.cordata J.Y.Zhang et al.)%乙醇痠氧化酶%全長cDNA%錶達分析
목내(Prunus salicina Lindl.var.cordata J.Y.Zhang et al.)%을순산양화매%전장cDNA%표체분석
Prunus salicina%glycolate oxidase%full-length cDNA%expression analysis
乙醇酸氧化酶（ glycolate oxidase，GLO）是植物光呼吸途径中的一种限速酶，催化羟乙酸盐氧化成乙醛酸盐和H2 O2，与植物的诱导抗病性密切相关。试验从已构建好的木奈（ Prunus salicina Lindl．var．cordata J．Y．Zhang et al．）5个不同生长发育时期叶片的均一化全长cDNA文库中分离得到了一个乙醇酸氧化酶（ GLO）基因，命名为PsGLO。序列分析表明，PsGLO基因cDNA全长为1531 bp，开放阅读框为1122 bp，编码374个氨基酸。氨基酸多重序列比对表明，该基因与陆地棉乙醇酸氧化酶基因相似性最高，为90％。荧光定量PCR分析表明， PsGLO基因在木奈叶中木奈叶芽、展开叶、幼叶、成熟叶、老叶5个不同生长发育时期中都有表达，其中，老叶中表达量最高，叶芽最低。
乙醇痠氧化酶（ glycolate oxidase，GLO）是植物光呼吸途徑中的一種限速酶，催化羥乙痠鹽氧化成乙醛痠鹽和H2 O2，與植物的誘導抗病性密切相關。試驗從已構建好的木奈（ Prunus salicina Lindl．var．cordata J．Y．Zhang et al．）5箇不同生長髮育時期葉片的均一化全長cDNA文庫中分離得到瞭一箇乙醇痠氧化酶（ GLO）基因，命名為PsGLO。序列分析錶明，PsGLO基因cDNA全長為1531 bp，開放閱讀框為1122 bp，編碼374箇氨基痠。氨基痠多重序列比對錶明，該基因與陸地棉乙醇痠氧化酶基因相似性最高，為90％。熒光定量PCR分析錶明， PsGLO基因在木奈葉中木奈葉芽、展開葉、幼葉、成熟葉、老葉5箇不同生長髮育時期中都有錶達，其中，老葉中錶達量最高，葉芽最低。
을순산양화매（ glycolate oxidase，GLO）시식물광호흡도경중적일충한속매，최화간을산염양화성을철산염화H2 O2，여식물적유도항병성밀절상관。시험종이구건호적목내（ Prunus salicina Lindl．var．cordata J．Y．Zhang et al．）5개불동생장발육시기협편적균일화전장cDNA문고중분리득도료일개을순산양화매（ GLO）기인，명명위PsGLO。서렬분석표명，PsGLO기인cDNA전장위1531 bp，개방열독광위1122 bp，편마374개안기산。안기산다중서렬비대표명，해기인여륙지면을순산양화매기인상사성최고，위90％。형광정량PCR분석표명， PsGLO기인재목내협중목내협아、전개협、유협、성숙협、로협5개불동생장발육시기중도유표체，기중，로협중표체량최고，협아최저。
Glycolate oxidase ( GLO) is a key enzyme in photorespiration and catalyzes the oxidation of gly-colate into glyoxylate with an equimolar amount of H2 O2 ,which is related with induction of disease resistance in plant.A clone,named PsGLO,was separated from cDNA library prepared from five different growth and de-velopment periods of leaf of Prunus salicina.Sequence analysis showed that PsGLO was 1 531 bp in length with a 1 122 nucleotides ORF that putatively encoded a protein with 374 amino acids.Multiple sequence alignments and phylogenetic tress analyses showde that GLO was clustered with Gossypium hirsutum GLO and showed high similarity (90%) in amino acid sequences.Quantitative real-time PCR analysis showed that the expression of PsGLO was observed in all different stages tested here including leafbud,unfoldedleaf,spire,climax leaf,old leaf and the highest expression level was found in old leaves,but the lowest in leafbud.
