CAJ | 학술논문

为克服采用传统方法分离木脂素的不足，采用高速逆流色谱法对珠子草中的木脂素进行分离，得到3个木脂素，通过红外光谱（ IR）、质谱（ MS）、核磁共振（ NMR）等方法鉴定为珠子草素（ niranthin）、叶下珠次素（hypophyllanthin）和叶下珠脂素（phyllanthin）。分离溶剂体系为正己烷/乙酸乙酯/甲醇/水（3/2/3/2，V/V），下相为流动相，流速为2 mL/min，转速为正转800 r/min，温度为25℃。实验结果为木脂素的分离提供了一条有效的途径。
위극복채용전통방법분리목지소적불족，채용고속역류색보법대주자초중적목지소진행분리，득도3개목지소，통과홍외광보（ IR）、질보（ MS）、핵자공진（ NMR）등방법감정위주자초소（ niranthin）、협하주차소（hypophyllanthin）화협하주지소（phyllanthin）。분리용제체계위정기완/을산을지/갑순/수（3/2/3/2，V/V），하상위류동상，류속위2 mL/min，전속위정전800 r/min，온도위25℃。실험결과위목지소적분리제공료일조유효적도경。
To overcome disadvantages of traditional lignans isolation methods,high-speed countercur-rent chromatography ( HSCCC) was employed and three lignans were isolated from Phyllanthus niru-ri Linn,which were identified as niranthin,hypophyllanthin,and phyllanthin by spectroscopic meth-ods,including infrared spectroscopy(IR),mass spectrometry(MS)and nuclear magnetic resonance ( NMR) .Separating processes were given as following:light phase as stationary phase and heavy phase as mobile phase from mixture of n-hexane,ethyl acetate,methanol and water(3/2/3/2,V/V) with flow rate 2 mL/min,rotation speed 800 r/min at 25℃.The results provide an effective way for isolation of lignans.