低频强噪声对大鼠听力的影响及内耳Caspase-3的表达
저빈강조성대대서은력적영향급내이Caspase-3적표체
Effects of High Level Low Frequency Noise on Hearing and Expression of Caspase-3 in Rats
  • 万方数据
    中华耳科学杂志 중화이과학잡지
    CHINESE JOURNAL OF OTOLOGY
    2014年 3期 503-506 ,共4页

    尹志利%吴玮%王刚%王鸿南%韩浩伦%李保卫%周丽斌%余萌%屈昌北%张建中%吴继华%曾新吾%龚昌超%田章福%柳珑

    윤지리%오위%왕강%왕홍남%한호륜%리보위%주려빈%여맹%굴창북%장건중%오계화%증신오%공창초%전장복%류롱

    低频噪声%耳蜗%大鼠%凋亡%Caspase-3 低頻譟聲%耳蝸%大鼠%凋亡%Caspase-3
    저빈조성%이와%대서%조망%Caspase-3
    Low frequency noise%Cochlea%Rat%Apoptosis%Caspase-3
    目的：观察高强度低频噪声对听力及内耳细胞凋亡的影响。方法52只大鼠随机分为正常对照组（Pr）13只和暴露组39只，暴露组包括即刻组（E0组）、3天组（E3组）和7天组（E7组）各13只，经强度为150dB中心频率在500Hz的稳态噪声持续暴露10分钟，分别于即刻、震后3天、7天，作脑干诱发电位检测，检测后处死动物，取材制作耳蜗石蜡切片行免疫组化观察。结果经150dB强噪声暴露后即刻，大鼠ABR阈值显著升高，3天组轻度恢复，7天组未进一步改善。暴露后各时间点ABR阈值较暴露前明显增高（P<0.01），E0组与E3组、E7组之间有统计学差异（P<0.05），E3组与E7组之间无统计学意义（P＞0.05）。免疫组化结果低频强声暴露后各组耳蜗Corti器、螺旋神经节细胞、血管纹和螺旋韧带均可见Caspase-3染色程度不同的阳性表达，对照组无明显表达，实验暴露组各时间点平均光密度值与对照组之间有统计学差异（P<0.01），E0组与E3组、E7组之间有统计学差异（P<0.05），E3组与E7组之间有统计学差异（P<0.05）。结论高强度的低频噪声能够造成听力明显损伤，但可部分恢复，Caspase-3介导的内耳细胞凋亡可能在噪声性耳聋的发病机制中起重要作用。
    목적：관찰고강도저빈조성대은력급내이세포조망적영향。방법52지대서수궤분위정상대조조（Pr）13지화폭로조39지，폭로조포괄즉각조（E0조）、3천조（E3조）화7천조（E7조）각13지，경강도위150dB중심빈솔재500Hz적은태조성지속폭로10분종，분별우즉각、진후3천、7천，작뇌간유발전위검측，검측후처사동물，취재제작이와석사절편행면역조화관찰。결과경150dB강조성폭로후즉각，대서ABR역치현저승고，3천조경도회복，7천조미진일보개선。폭로후각시간점ABR역치교폭로전명현증고（P<0.01），E0조여E3조、E7조지간유통계학차이（P<0.05），E3조여E7조지간무통계학의의（P＞0.05）。면역조화결과저빈강성폭로후각조이와Corti기、라선신경절세포、혈관문화라선인대균가견Caspase-3염색정도불동적양성표체，대조조무명현표체，실험폭로조각시간점평균광밀도치여대조조지간유통계학차이（P<0.01），E0조여E3조、E7조지간유통계학차이（P<0.05），E3조여E7조지간유통계학차이（P<0.05）。결론고강도적저빈조성능구조성은력명현손상，단가부분회복，Caspase-3개도적내이세포조망가능재조성성이롱적발병궤제중기중요작용。
    Objective To study the effect of high intensity low frequency noise on hearing and apoptosis of inner ear cells. Methods Rats (n=52 ) were randomly divided into a control group (Pr) (n=13), and three noise exposure groups (n=39) that were examined immediately (E0 group, n=13), three days (E3 group, n=13) or 7 days (E7 group, n=13) following exposure to a 150 dB SPL steady noise centered around 500 Hz for 10 minutes, respectively, The auditory brainstem response was record-ed, followed by cochlear morphology examination after the animals were sacrificed and cochlear paraffin sections were prepared and stained using immunohistochemical techniques. Results Immediately after exposure to the 150 dB SPL noise, ABR thresholds were significantly elevated. Moderate recovery was seen at 3 days after exposure, with little further improvement at 7 days after exposure. ABR thresholds at all time points following exposure were higher than before exposure (P<0.01) ABR thresholds showed significant differences between the E0 group and E3 and E7 groups (P<0.05), but not between the E3 and E7 groups (P>0.05). Immunohistochemistry results showed various levels of caspase-3 expression in the Organ of Corti, spiral gan-glion cells, stria vascularis and spiral ligaments in the low-frequency sound exposure groups but not in the control group. The average optical density was different between the control and exposure groups, among the E0, E3 and E7 groups (P<0.01) and between the E3 and E7 groups (P<0.01). There was no significant caspase-3 expression in the control group. Conclusion High-intensity low-frequency noise can cause significant hearing damage, which can later partially recover. Caspase-3-medi-ated inner ear apoptosis may play an important role in the pathogenesis of noise-induced hearing loss.
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