CAJ | 학술논문

应用液质联用技术UPLC/Q-TOF-MS建立一种有效快速分析鉴定毛茛生物碱类成分的方法并研究其质谱裂解规律。采用液质联用技术UPLC/Q-TOF-MS ，根据毛茛生物碱类成分的一级质谱数据，二级特征裂解碎片及标准品比对鉴定毛茛生物碱类成分。共鉴定了12种生物碱类成分，并探讨了其裂解规律。首次从毛茛中分离鉴定了12种生物碱类物质，建立了一种有效的基于UPLC/Q-TOF-MS鉴定毛茛生物碱类成分的方法。
응용액질련용기술UPLC/Q-TOF-MS건립일충유효쾌속분석감정모간생물감류성분적방법병연구기질보렬해규률。채용액질련용기술UPLC/Q-TOF-MS ，근거모간생물감류성분적일급질보수거，이급특정렬해쇄편급표준품비대감정모간생물감류성분。공감정료12충생물감류성분，병탐토료기렬해규률。수차종모간중분리감정료12충생물감류물질，건립료일충유효적기우UPLC/Q-TOF-MS감정모간생물감류성분적방법。
To establish a rapid and effective method for analysis and identification of the alkaloids from Ranunculus japoni-cus Thunb by ultra-performance liquid chromatogaraphy with quadruple-time-of-flight mass spectrometry (UPLC/Q-TOF-MS) and discuss their fragmentation regularity ,the UPLC/Q-TOF-MS was used to identify the alkaloids from Ranunculus japonicus Thunb by their MS data ,tandem characteristic fragment ions and standards .In the end ,12 alkaloids were identi-fied from Ranunculus japonicus for the first time ,and their fragmentation regularity was discussed .Thus ,a rapid and effec-tive analysis and identification method for the alkaloids from Ranunculus japonicus by UPLC/Q-TOF-MS is established .