CAJ | 학술논문

以黑加仑品种世纪光和世纪星为材料,盆栽扦插幼苗分别经0,50,100,150和200 mmol/L NaCl 浓度处理15 d,在胁迫处理第5天、第10天和第15天取样并测定叶片游离脯氨酸、可溶性糖、丙二醛(MDA)含量及质膜相对透性.结果表明,随 NaCl 浓度增大和胁迫时间增加,世纪光体内游离脯氨酸和可溶性糖含量一直呈上升趋势,而世纪星在第5天至第10天游离脯氨酸和可溶性糖含量虽有所增加,但增加幅度远低于世纪光,在第15天各处理浓度的含量则开始下降,150,200 mmol/L 浓度处理下的含量,相比 CK 分别下降了7．8％,16．6％和3．4％,20．0％；两个品种的质膜相对透性和 MDA 含量也随 NaCl 胁迫时间和处理浓度的增加而增加,世纪光经200 mmol/L NaCl 浓度处理15 d 后,质膜相对透性未超过50％,而世纪星经150 mmol/L 浓度处理15 d 时其质膜相对透性就超过了50％,达到了半致死程度.说明黑加仑世纪光在 NaCl 胁迫环境下具有较强的有机渗透调节能力,其耐盐性强于世纪星.
이흑가륜품충세기광화세기성위재료,분재천삽유묘분별경0,50,100,150화200 mmol/L NaCl 농도처리15 d,재협박처리제5천、제10천화제15천취양병측정협편유리포안산、가용성당、병이철(MDA)함량급질막상대투성.결과표명,수 NaCl 농도증대화협박시간증가,세기광체내유리포안산화가용성당함량일직정상승추세,이세기성재제5천지제10천유리포안산화가용성당함량수유소증가,단증가폭도원저우세기광,재제15천각처리농도적함량칙개시하강,150,200 mmol/L 농도처리하적함량,상비 CK 분별하강료7．8％,16．6％화3．4％,20．0％；량개품충적질막상대투성화 MDA 함량야수 NaCl 협박시간화처리농도적증가이증가,세기광경200 mmol/L NaCl 농도처리15 d 후,질막상대투성미초과50％,이세기성경150 mmol/L 농도처리15 d 시기질막상대투성취초과료50％,체도료반치사정도.설명흑가륜세기광재 NaCl 협박배경하구유교강적유궤삼투조절능력,기내염성강우세기성.
In this study,Ribes nigrum L.varieties Century Light and the Century Star were used as ex-perimental materials,potted cutting seedlings were treated with 0,50,100,150 and 200 mmol/L NaCl con-centration for 15 d.On the 5th,10th and 15th after stress treatment,sample was taken and free proline,sol-uble sugar,malondialdehyde (MDA)content and the relative permeability of plasma membrane were meas-ured.The results showed that with the increase of NaCl concentration and stress time,the free proline and soluble sugar content of Century Light has been an upward trend,and the free proline and soluble sugar content of Century Star has been increased on the 5th-10thd,but the increase was much lower than the Century Light,on the 15thd content of each treatment concentration began to decline,150,200 mmol/L concentration were decreased,compared with CK by 7.8%,1 6.6% and 3.4%,20.0%;the relative permeabil-ity of plasma membrane and MDA content of the two varieties also increased with the increase of NaCl stress days and concentration,and the Century Star increase was far greater than the Century Light by 200 mmol/L concentration of NaCl after 15d treatment,the relative permeability of plasma membrane in-creased by no more than 50%,when the 150 mmol/L concentrations of Century Star were treated for 15 days,the relative permeability of plasma membrane increased by more than 50%,which reached the semi lethal degree,which showed that Ribes nigrum L.varieties Century Light has stronger organic osmot-ic adjustment ability under NaCl stress conditions,its salt tolerance was better than the Century Star.