中医药学报
中醫藥學報
중의약학보
ACTA CHINESE MEDICINE AND PHARMACOLOGY
2014年
5期
71-75
,共5页
莫雨平%姚海江%宋洪涛%陈万顺%王鑫%加吾拉·阿不力孜%许安萍%唐银杉%韩向博%李志刚
莫雨平%姚海江%宋洪濤%陳萬順%王鑫%加吾拉·阿不力孜%許安萍%唐銀杉%韓嚮博%李誌剛
막우평%요해강%송홍도%진만순%왕흠%가오랍·아불력자%허안평%당은삼%한향박%리지강
脉冲电针%音乐电针%慢性应激%抑郁症%大鼠%下丘脑%促甲状腺激素释放激素
脈遲電針%音樂電針%慢性應激%抑鬱癥%大鼠%下丘腦%促甲狀腺激素釋放激素
맥충전침%음악전침%만성응격%억욱증%대서%하구뇌%촉갑상선격소석방격소
Pulse electro -acupuncture%Music electro-acupuncture%Chronic unpredictable mild stress%Depression%Rat%Hypothalamus%Thyrotropin releasing hormone
目的:观察不同电针对慢性应激抑郁模型( CUMS)大鼠下丘脑中促甲状腺激素释放激素( TRH)表达的影响,探讨TRH在大鼠实验性抑郁症发病过程中的作用机制及不同电针干预对其的影响。方法:将60只清洁健康雄性SD大鼠,随机分为正常组、模型组、脉冲电针组、音乐电针组及氟西汀组,每组12只。孤养结合CUMS21天复制抑郁症模型,通过开野试验观察大鼠行为学变化,采用免疫组织化学法、酶联免疫吸附法及荧光实时定量PCR分别检测TRH在大鼠下丘脑中的表达情况。结果:模型组大鼠开野实验水平运动及垂直运动明显减少,脉冲电针组、音乐电针组及氟西汀组均可改善此变化;模型组大鼠下丘脑内TRH表达均明显降低,脉冲电针组、音乐电针组及氟西汀组可逆转此病理变化,但氟西汀组治疗效果较两组电针治疗效果略胜一筹;两组电针治疗之间无明显统计学差异。结论:抑郁大鼠的TRH含量低于正常;电针治疗抑郁症可能是通过升高TRH的含量而发挥抗抑郁作用。音乐电针与脉冲电针治疗抑郁症效果差异不明显。
目的:觀察不同電針對慢性應激抑鬱模型( CUMS)大鼠下丘腦中促甲狀腺激素釋放激素( TRH)錶達的影響,探討TRH在大鼠實驗性抑鬱癥髮病過程中的作用機製及不同電針榦預對其的影響。方法:將60隻清潔健康雄性SD大鼠,隨機分為正常組、模型組、脈遲電針組、音樂電針組及氟西汀組,每組12隻。孤養結閤CUMS21天複製抑鬱癥模型,通過開野試驗觀察大鼠行為學變化,採用免疫組織化學法、酶聯免疫吸附法及熒光實時定量PCR分彆檢測TRH在大鼠下丘腦中的錶達情況。結果:模型組大鼠開野實驗水平運動及垂直運動明顯減少,脈遲電針組、音樂電針組及氟西汀組均可改善此變化;模型組大鼠下丘腦內TRH錶達均明顯降低,脈遲電針組、音樂電針組及氟西汀組可逆轉此病理變化,但氟西汀組治療效果較兩組電針治療效果略勝一籌;兩組電針治療之間無明顯統計學差異。結論:抑鬱大鼠的TRH含量低于正常;電針治療抑鬱癥可能是通過升高TRH的含量而髮揮抗抑鬱作用。音樂電針與脈遲電針治療抑鬱癥效果差異不明顯。
목적:관찰불동전침대만성응격억욱모형( CUMS)대서하구뇌중촉갑상선격소석방격소( TRH)표체적영향,탐토TRH재대서실험성억욱증발병과정중적작용궤제급불동전침간예대기적영향。방법:장60지청길건강웅성SD대서,수궤분위정상조、모형조、맥충전침조、음악전침조급불서정조,매조12지。고양결합CUMS21천복제억욱증모형,통과개야시험관찰대서행위학변화,채용면역조직화학법、매련면역흡부법급형광실시정량PCR분별검측TRH재대서하구뇌중적표체정황。결과:모형조대서개야실험수평운동급수직운동명현감소,맥충전침조、음악전침조급불서정조균가개선차변화;모형조대서하구뇌내TRH표체균명현강저,맥충전침조、음악전침조급불서정조가역전차병리변화,단불서정조치료효과교량조전침치료효과략성일주;량조전침치료지간무명현통계학차이。결론:억욱대서적TRH함량저우정상;전침치료억욱증가능시통과승고TRH적함량이발휘항억욱작용。음악전침여맥충전침치료억욱증효과차이불명현。
Objective:To investigate the influence on the expression of TRH in the hypothalamus of rats with chronic un-predictable mild stress by different electro-acupuncture( EA) treatments, to elucidate the possible role of TRH in the development of experimental depression and the effect of different EA for them.Methods:Adult male SD rats were ran-domly divided into a normal control group(n=12),a model group (n=12),a pulsed EA group(n=12),a music EA group( n=12) and a fluoxetine group( n=12) .Depression models were established by lonely raising and chronic unpre-dictable mild stress for 21 days.Open-field test was performed to test the behavior of rats.The expression of hypothala-mus TRH was detected by using immunohistochemistry , enzyme linked immunosorbent assay( ELISA) and real-time PCR techniques respectively.Results:The cross and rear scores of the model group were significantly lower than those in the normal control group, while pulsed EA group, music EA group and fluoxetine group could reverse it.The expres-sion of hypothalamus TRH in model group decreased significantly,and the three kinds of treatment could improve these pathological change, but the effect of fluoxetine was better than other two EA groups;in addition, there was no statistical difference between the effects of pulse-EA and music-EA treatments.Conclusion:The levels of TRH in hypothalamus of depression rats are lower than normal.EA treatments may have antagonism against depression by increasing the levels of TRH in hypothalamus,and there is no difference between the effects of pulsed EA treatment and music EA treatment on depression.