CAJ | 학술논문

水稻秸秆饲料化利用是水稻秸秆综合开发利用的重要途径之一,但水稻秸秆中的农药残留可能会影响动物饲喂安全。吡虫啉和三环唑是水稻生产中广泛应用的杀虫剂和杀菌剂。本研究选用南粳5055和镇稻10号的水稻秸秆为试验材料,利用乙腈提取水稻秸秆中的吡虫啉和三环唑,经ENVI-18小柱和分散固相吸附剂PSA净化,采用高效液相色谱法分析,检测水稻秸秆中的农药残留量及其变化趋势,为水稻秸秆饲料化利用的安全性提供依据。结果表明,吡虫啉和三环唑的添加回收率为88.36%~119.98%,变异系数为1.83%~12.38%,吡虫啉和三环唑的最小检出量分别为1.00×10-9 g和1.25×10-10 g。吡虫啉在南粳5055和镇稻10号中的半衰期分别为2.082 d和1.905 d,三环唑在南粳5055和镇稻10号的半衰期分别为6.086 d和5.660 d。吡虫啉喷施30 d后,残留量小于0.3070 mg/kg,三环唑喷施35 d后,残留量小于0.0561 mg/kg,供试水稻秸秆可安全用作饲料。
수도갈간사료화이용시수도갈간종합개발이용적중요도경지일,단수도갈간중적농약잔류가능회영향동물사위안전。필충람화삼배서시수도생산중엄범응용적살충제화살균제。본연구선용남갱5055화진도10호적수도갈간위시험재료,이용을정제취수도갈간중적필충람화삼배서,경ENVI-18소주화분산고상흡부제PSA정화,채용고효액상색보법분석,검측수도갈간중적농약잔류량급기변화추세,위수도갈간사료화이용적안전성제공의거。결과표명,필충람화삼배서적첨가회수솔위88.36%~119.98%,변이계수위1.83%~12.38%,필충람화삼배서적최소검출량분별위1.00×10-9 g화1.25×10-10 g。필충람재남갱5055화진도10호중적반쇠기분별위2.082 d화1.905 d,삼배서재남갱5055화진도10호적반쇠기분별위6.086 d화5.660 d。필충람분시30 d후,잔류량소우0.3070 mg/kg,삼배서분시35 d후,잔류량소우0.0561 mg/kg,공시수도갈간가안전용작사료。
Using straw as fodder for herbivorous animal has been an important approach to the comprehensive utiliza-tion of crops. However, application of pesticides and bactericides during rice growth period may lead to high residues in rice straw. Imidacloprid and tricyclazole are pesticide and bactericide commonly used in rice production. In this study, two rice varieties Nanjing 5055 and Zhendao 10 were selected to detect the residues and degradation dynamics of imidacloprid and tricyclazole in the straw. Imidacloprid and tricyclazole were extracted with acetonitrile, purified with ENVI-18 SPE and dispersive solid-phase extraction, and determined by reversed phase column HPLC equipped with UVD. The results showed that the added recoveries of imidacloprid and tricyclazole were between 88. 36% and 119. 98%, with the coefficients of var-iation between 1. 83% and 12. 38%. The minimal detectable ammount of imidacloprid and tricyclazole were 1. 00×10-9 g and 1. 25× 10-10 g, respectively. The half-life of degradation for imidacloprid were 2. 082 d in Nanjing 5055 and 2. 082 d in Zhendao 10, respectively, and were 6. 086 d and 5. 66 d for tricyclazole. The residues were less than 0. 307 0 mg/kg for imida cloprid after 30-d application and less than 0. 056 1 mg/kg for tricyclazole, which met the safety standards for animal fodder.