CAJ | 학술논문

为了提高双孢蘑菇多糖的提取得率和效率，并研究双孢蘑菇多糖的组分，在考察了单因素水料比、超声功率、超声时间对双孢蘑菇子实体多糖提取得率影响的基础上，采用 Box-Behnken响应面设计法，建立了3个影响因素与双孢蘑菇子实体多糖提取得率的回归方程，优化提取工艺。同时利用 DEAE Sepharose FF阴离子层析法对多糖进行了分离。 Design Expert 软件分析结果表明：在水料比36：1、超声功率594 W、超声时间17 min的优化工艺条件下，双孢蘑菇子实体多糖的提取得率可高达6.63%。分离出2个多糖组分，分子量分别为2.75×105和1.4×104。通过响应面法设计结合超声波辅助提取能提高提取效率、缩短提取时间和保护有效成分，具有一定实际应用价值。 DEAE Sepharose FF层析柱对双孢蘑菇多糖具有良好的分离效果。
위료제고쌍포마고다당적제취득솔화효솔，병연구쌍포마고다당적조분，재고찰료단인소수료비、초성공솔、초성시간대쌍포마고자실체다당제취득솔영향적기출상，채용 Box-Behnken향응면설계법，건립료3개영향인소여쌍포마고자실체다당제취득솔적회귀방정，우화제취공예。동시이용 DEAE Sepharose FF음리자층석법대다당진행료분리。 Design Expert 연건분석결과표명：재수료비36：1、초성공솔594 W、초성시간17 min적우화공예조건하，쌍포마고자실체다당적제취득솔가고체6.63%。분리출2개다당조분，분자량분별위2.75×105화1.4×104。통과향응면법설계결합초성파보조제취능제고제취효솔、축단제취시간화보호유효성분，구유일정실제응용개치。 DEAE Sepharose FF층석주대쌍포마고다당구유량호적분리효과。
To improve the yield and extraction efficiency of polysaccharides from white button mushroom ( Agari-cus bisporus) , ultrasonic-assisted extraction ( UAE) was applied for polysaccharides extraction and the three parame-ters, the ratio of water volume to raw material weight ( W/M ratio) , ultrasonic power, and extraction time were stud-ied and optimized by response surface methodology ( RSM). The highest polysaccharides yield of 6. 63% was a-chieved. When W/M ratio was 36 : 1, ultrasonic power was 594 W and extraction time was 17 min. Two polysaccha-ride fractions ( AⅠ and AⅡ) were extracted ion exchange chromatography of DEAE Sepharose F F, with the molecu-lar weights of 2. 75×105 and 1. 4×104, respectively. Response surface methodology is efficient and practical for im-proving the efficiency of ultrasonic-assisted extraction of polysaccharides from white button mushroom by shortening ex-traction time duration and protecting the effective contents of polysaccharides.