CAJ | 학술논문

为了研究福瑞鲤不同家系的遗传多样性,利用TRAP分子标记对生长速度有差异的家系(4个生长速度快,4个生长速度慢)进行了遗传分析。结果显示：8个家系的多态性条带数在9.41至12.00之间,多态性比率范围为52.04%~66.02%,多态信息含量为0.1853~0.2430, Nei 遗传多样指数和 Shannon 信息指数分别为0.1875~0.2410和0.2780~0.3510,其中家系85#的各遗传参数值最高,而家系74#的最低。家系间的遗传分化指数(Fst)及分子变异方差(AMOVA)分析结果显示,除了家系56#与92#(Fst=0.0516)及51#与89#(Fst=0.0956)这2对家系间处于中等程度的遗传分化(0.05≤Fst≤0.15)之外,其他家系间遗传分化水平均较低(Fst<0.05),群体的总遗传变异主要来源于家系内个体间的遗传差异,其方差分量的贡献率达98.3%。遗传距离分析结果表明,家系26#与92#的遗传距离最大,家系85#与89#的遗传距离最小；基于此的UPGMA聚类分析结果显示,家系56#与74#以及家系85#与89#的亲缘关系比较近,聚类在同一分支上,而其他家系亲缘关系相对较远。研究结果说明8个福瑞鲤家系内遗传变异较大,遗传多样性水平较高,具有一定的育种潜力,可扩大福瑞鲤的选育空间。
위료연구복서리불동가계적유전다양성,이용TRAP분자표기대생장속도유차이적가계(4개생장속도쾌,4개생장속도만)진행료유전분석。결과현시：8개가계적다태성조대수재9.41지12.00지간,다태성비솔범위위52.04%~66.02%,다태신식함량위0.1853~0.2430, Nei 유전다양지수화 Shannon 신식지수분별위0.1875~0.2410화0.2780~0.3510,기중가계85#적각유전삼수치최고,이가계74#적최저。가계간적유전분화지수(Fst)급분자변이방차(AMOVA)분석결과현시,제료가계56#여92#(Fst=0.0516)급51#여89#(Fst=0.0956)저2대가계간처우중등정도적유전분화(0.05≤Fst≤0.15)지외,기타가계간유전분화수평균교저(Fst<0.05),군체적총유전변이주요래원우가계내개체간적유전차이,기방차분량적공헌솔체98.3%。유전거리분석결과표명,가계26#여92#적유전거리최대,가계85#여89#적유전거리최소；기우차적UPGMA취류분석결과현시,가계56#여74#이급가계85#여89#적친연관계비교근,취류재동일분지상,이기타가계친연관계상대교원。연구결과설명8개복서리가계내유전변이교대,유전다양성수평교고,구유일정적육충잠력,가확대복서리적선육공간。
To reveal the diversity of differently developed FFRC strain common carp ( Cyprinus carpio) , target region amplified polymorphism(TRAP) molecular markers were used to analyze 8 families (four families grow fast and other four grow slowly). The results show that the number of polymorphism bands of the eight families ranged from 9. 41 to 12. 00, the polymorphism percentage ranged from 52. 04% to 66. 02%, the polymorphism information content ( PIC ) ranged from 0. 185 3 to 0. 243 0, and the Nei’s genetic diversity and Shannon’s indexes ranged from 0. 187 5 to 0. 241 0 and from 0. 278 0 to 0. 351 0, respectively. Family 85# had the highest genetic parameter values, while 74# had the lowest. Pairwise Fst(genetic differentiation index) and AMOVA a-nalysis showed that except for 56# and 92#(Fst=0.051 6) as well as 51#and 89#(Fst=0. 095 6) in moderate differen-tiation(0. 05≤Fst≤0. 15), the rest of the families were in lower genetic differentiation (Fst<0. 05). The majority of genetic variability was within family (98. 3%), rather than among family (1. 7%). The genetic distance between 26#and 92# was farthest while the genetic distance between 85#and 89# was the lowest. The UPGMA phylogenetic tree of the eight families constructed based on Nei’s genetic distance clustered 56# and 74# together, and 85#and 89#. The findings suggested that the eight families had a large selection potential due to the great genet-ic variation and high level of genetic diversity.