华西口腔医学杂志
華西口腔醫學雜誌
화서구강의학잡지
WEST CHINA JOURNAL OF STOMATOLOGY
2014年
5期
509-512
,共4页
尚针针%李欣%孙惠强%贾可丽
尚針針%李訢%孫惠彊%賈可麗
상침침%리흔%손혜강%가가려
MC3T3-E1细胞%流体剪切力%信号通路
MC3T3-E1細胞%流體剪切力%信號通路
MC3T3-E1세포%류체전절력%신호통로
MC3T3-E1 cell%fluid shear stress%signal pathway
目的：探讨适宜流体剪切力力值及作用时间下成骨细胞差异表达基因及相关信号通路。方法通过平行板流室加力装置对盖玻片培养的MC3T3-E1细胞施加流体剪切力，提取总RNA，进行包括44170个基因的全功能组表达谱基因芯片检测，对差异表达基因进行Pathway和GO分析，并采用实时荧光定量逆转录聚合酶链反应（RT-PCR）对芯片结果进行验证。结果芯片结果显示，差异基因共有884个，其中表达增强基因444个，表达降低基因440个。Pathway分析涉及的信号通路，主要包括Notch信号通路、RIG-Ⅰ样受体信号通路等。GO分析主要涉及前列腺素的生物合成、一氧化氮介导的信号传导、钙介导的信号及细胞免疫反应等不同的功能分类。结论流体剪切力对MC3T3-E1细胞的保护作用可能与激活促进细胞生存及抑制细胞凋亡相关信号通路及生物学过程相关。
目的：探討適宜流體剪切力力值及作用時間下成骨細胞差異錶達基因及相關信號通路。方法通過平行闆流室加力裝置對蓋玻片培養的MC3T3-E1細胞施加流體剪切力，提取總RNA，進行包括44170箇基因的全功能組錶達譜基因芯片檢測，對差異錶達基因進行Pathway和GO分析，併採用實時熒光定量逆轉錄聚閤酶鏈反應（RT-PCR）對芯片結果進行驗證。結果芯片結果顯示，差異基因共有884箇，其中錶達增彊基因444箇，錶達降低基因440箇。Pathway分析涉及的信號通路，主要包括Notch信號通路、RIG-Ⅰ樣受體信號通路等。GO分析主要涉及前列腺素的生物閤成、一氧化氮介導的信號傳導、鈣介導的信號及細胞免疫反應等不同的功能分類。結論流體剪切力對MC3T3-E1細胞的保護作用可能與激活促進細胞生存及抑製細胞凋亡相關信號通路及生物學過程相關。
목적：탐토괄의류체전절력력치급작용시간하성골세포차이표체기인급상관신호통로。방법통과평행판류실가력장치대개파편배양적MC3T3-E1세포시가류체전절력，제취총RNA，진행포괄44170개기인적전공능조표체보기인심편검측，대차이표체기인진행Pathway화GO분석，병채용실시형광정량역전록취합매련반응（RT-PCR）대심편결과진행험증。결과심편결과현시，차이기인공유884개，기중표체증강기인444개，표체강저기인440개。Pathway분석섭급적신호통로，주요포괄Notch신호통로、RIG-Ⅰ양수체신호통로등。GO분석주요섭급전렬선소적생물합성、일양화담개도적신호전도、개개도적신호급세포면역반응등불동적공능분류。결론류체전절력대MC3T3-E1세포적보호작용가능여격활촉진세포생존급억제세포조망상관신호통로급생물학과정상관。
Objective To explore the differentially expressed genes and related signaling pathways in MC3T3-E1 osteo-blasts in response to suitable fluid shear stress values and action time with cDNA microarrays. Methods MC3T3-E1 cells cultured on a cover slip were subjected to fluid shear stress using a parallel plate flow chamber. The harvested RNA was used for microarray hybridization comprising approximately 44 170 genes, as well as for the subsequent real-time quantitative polymerase chain reaction validation of expression levels for selected genes. Microarray results were analyzed by using both GO and Pathway analysis. Results Microarray analysis indicated that 884 differentially expressed genes were found. Among these genes, 444 were upregulated, whereas 440 were downregulated. The Notch signal and RIG-Ⅰ-like receptor signaling pathways were involved in the Pathway analysis. GO analysis mainly involved different functional classifications, such as prostaglandin biosynthesis, nitric oxide-mediated signal transduction, calcium mediated signal, and cellular immune response, among others. Conclusion The mechanism underlying the protective effect of fluid shear stress on MC3T3-E1 cells might be related to promoting cell survival- and inhibiting cell apoptosis-related signaling pathways and biological processes.
