华西口腔医学杂志
華西口腔醫學雜誌
화서구강의학잡지
WEST CHINA JOURNAL OF STOMATOLOGY
2014年
5期
455-458
,共4页
盛海莹%左艳萍%陈潇%苑学微
盛海瑩%左豔萍%陳瀟%苑學微
성해형%좌염평%진소%원학미
下颌功能前伸%髁突软骨%磷脂酶C-γ1tyr783%骨改建
下頜功能前伸%髁突軟骨%燐脂酶C-γ1tyr783%骨改建
하합공능전신%과돌연골%린지매C-γ1tyr783%골개건
functional mandibular protraction%condylar cartilage%phospholipase C-gammaltyr783%bone remodeling
目的:研究大鼠下颌功能前伸后髁突软骨中磷脂酶C-γ1tyr783(PLC-γ1tyr783)表达及其变化规律,探讨PLC-γ1tyr783在功能矫治前伸大鼠下颌髁突软骨骨改建中的作用,为临床上骨骼矫形治疗提供实验依据。方法选取4周龄健康雄性Sprague-Dawley(SD)大鼠60只,适应性饲养1周后随机等量分为实验组和对照组,实验组每天24h佩戴自制下颌前伸斜面导板矫治器,对照组不戴矫治器。2组大鼠分别于戴用矫治器1、3、7、14、21、28d时各断颈处死5只,取双侧髁突,常规固定、脱钙、脱水、包埋。石蜡切片采用免疫组织化学方法结合图像分析检测PLC-γ1tyr783在髁突软骨中的表达分布及其变化规律。结果对照组PLC-γ1tyr783表达水平随着实验周期的推移逐渐减弱(P>0.05),实验组大鼠髁突软骨处PLC-γ1tyr783的表达在第14天时达到高峰,与对照组相比差异有统计学意义(P<0.01)。结论 PLC-γ1tyr783参与了下颌功能前伸大鼠髁突软骨的骨改建。
目的:研究大鼠下頜功能前伸後髁突軟骨中燐脂酶C-γ1tyr783(PLC-γ1tyr783)錶達及其變化規律,探討PLC-γ1tyr783在功能矯治前伸大鼠下頜髁突軟骨骨改建中的作用,為臨床上骨骼矯形治療提供實驗依據。方法選取4週齡健康雄性Sprague-Dawley(SD)大鼠60隻,適應性飼養1週後隨機等量分為實驗組和對照組,實驗組每天24h珮戴自製下頜前伸斜麵導闆矯治器,對照組不戴矯治器。2組大鼠分彆于戴用矯治器1、3、7、14、21、28d時各斷頸處死5隻,取雙側髁突,常規固定、脫鈣、脫水、包埋。石蠟切片採用免疫組織化學方法結閤圖像分析檢測PLC-γ1tyr783在髁突軟骨中的錶達分佈及其變化規律。結果對照組PLC-γ1tyr783錶達水平隨著實驗週期的推移逐漸減弱(P>0.05),實驗組大鼠髁突軟骨處PLC-γ1tyr783的錶達在第14天時達到高峰,與對照組相比差異有統計學意義(P<0.01)。結論 PLC-γ1tyr783參與瞭下頜功能前伸大鼠髁突軟骨的骨改建。
목적:연구대서하합공능전신후과돌연골중린지매C-γ1tyr783(PLC-γ1tyr783)표체급기변화규률,탐토PLC-γ1tyr783재공능교치전신대서하합과돌연골골개건중적작용,위림상상골격교형치료제공실험의거。방법선취4주령건강웅성Sprague-Dawley(SD)대서60지,괄응성사양1주후수궤등량분위실험조화대조조,실험조매천24h패대자제하합전신사면도판교치기,대조조불대교치기。2조대서분별우대용교치기1、3、7、14、21、28d시각단경처사5지,취쌍측과돌,상규고정、탈개、탈수、포매。석사절편채용면역조직화학방법결합도상분석검측PLC-γ1tyr783재과돌연골중적표체분포급기변화규률。결과대조조PLC-γ1tyr783표체수평수착실험주기적추이축점감약(P>0.05),실험조대서과돌연골처PLC-γ1tyr783적표체재제14천시체도고봉,여대조조상비차이유통계학의의(P<0.01)。결론 PLC-γ1tyr783삼여료하합공능전신대서과돌연골적골개건。
Objective To investigate the changes in the expression of phospholipase C-gammaltyr783 (PLC-γ1tyr783) in the condylar cartilage of a young rat during functional mandibular protraction. This work also explores the function of PLC-γ1tyr783 in the rat mandibular condylar cartilage bone remodeling, which could provide experimental evidence for clinical bone ortho-pedic work. Methods A total of 60 four-week-old male Sprague-Dawley (SD) rats were used in this study. The rats were divided equally and randomly into experimental group and control group. The functional appliances that were fitted to the upper incisors of the animals in the experimental group were worn 24 h a day after the rats were fed for 7 d with homemade pellet feed. The animals in the experimental group, along with their matched controls, were sacrificed after 1, 3, 7, 14, 21, and 28 d. The bilateral condylar was fixed, decalcified, dehyded, and then conventional paraffin embedded. Immunohisto-chemistry of PLC-γ1tyr783 was applied to observe its express distribution and variation. Results The expression of PLC-γ1tyr783 decreased gradually in the control group, which showed age-related changes (P>0.05). On the 14th day, PLC-γ1tyr783 expres-sion in the experimental group was significantly higher than that in the control group. PLC-γ1tyr783 expression began to appear statistically and significantly different between the two groups (P<0.01). Conclusion PLC-γ1tyr783 is involved in the bone remodeling process of the rat condylar cartilage after functional mandibular protraction.