CAJ | 학술논문

为了探索提取土壤DNA之前去除腐殖酸的方法，采用芬顿试剂对土壤样品进行预处理，结果表明芬顿试剂可以有效地去除土壤中的腐殖酸，未经处理和经芬顿试剂处理20～100 min的土壤，均可提取到接近23 kb大小的宏基因组DNA。16S rDNA PCR扩增表明，40～100 min处理土壤提取的DNA可以成功扩增到目的片段，而处理20 min的土壤不一定能扩增到目的片段，未经处理的土壤则不能扩增到目的片段。芬顿试剂可以用于土壤预处理以去除腐殖酸。
위료탐색제취토양DNA지전거제부식산적방법，채용분돈시제대토양양품진행예처리，결과표명분돈시제가이유효지거제토양중적부식산，미경처리화경분돈시제처리20～100 min적토양，균가제취도접근23 kb대소적굉기인조DNA。16S rDNA PCR확증표명，40～100 min처리토양제취적DNA가이성공확증도목적편단，이처리20 min적토양불일정능확증도목적편단，미경처리적토양칙불능확증도목적편단。분돈시제가이용우토양예처리이거제부식산。
In order to investigate the methods to remove humic acid before DNA extraction from soils, Fenton reagent was employed to carry on pretreatment to soil samples. It showed that metagenome DNA was extracted successfully from all the soils, including the soils untreated and treated for 20 to 100 minutes by Fenton reagent. All the metagenome DNA are close to 23 kb in size. The 16S rDNA fragments were ampliifed successfully based on all the template DNA from soils treated for 40 to 100 minutes, while unsuccessfully for only 20 minutes. Ampliifcation was not obtained for the template DNA from the soils untreated. Therefore, Fenton reagent can be applied to remove humic acid from soils.