华南农业大学学报
華南農業大學學報
화남농업대학학보
JOURNAL OF SOUTH CHINA AGRICULTURAL UNIVERSITY
2014年
6期
89-93
,共5页
尾叶桉%种质资源%核心种质
尾葉桉%種質資源%覈心種質
미협안%충질자원%핵심충질
Eucalyptus urophylla%germplasm%core collection
【目的】为了快速、准确地从丰富的尾叶桉Eucalyptus urophylla种质资源中鉴定出育种上迫切需要的优异基因,初步构建了尾叶桉核心种质,以便简化管理,提高遗传资源在尾叶桉改良中的利用效率。【方法】以尾叶桉27个种源194个家系的树高、胸径、材积等17个数量性状为依据,采用不同的取样比例(5%、10%、20%、30%和40%)、3种遗传距离计算方法(欧氏距离、标准欧氏距离和马氏距离)、4种系统聚类方法(最短距离法、最长距离法、不加权类平均法和离差平方和法)和2种取样方法(随机取样法和最小距离逐步取样法)构建尾叶桉核心种质资源库,利用均值差异百分率(MD)、方差差异百分率(VD)、变异系数变化率(VR)和极差符合率(CR)作为构建核心种质的评价指标。【结果和结论】结果表明采用10%的抽样比例、标准欧氏距离、最短距离系统聚类法和最小距离逐步取样法构建的包括25个种源100个家系的尾叶桉核心种质最能代表原有的种质群体。
【目的】為瞭快速、準確地從豐富的尾葉桉Eucalyptus urophylla種質資源中鑒定齣育種上迫切需要的優異基因,初步構建瞭尾葉桉覈心種質,以便簡化管理,提高遺傳資源在尾葉桉改良中的利用效率。【方法】以尾葉桉27箇種源194箇傢繫的樹高、胸徑、材積等17箇數量性狀為依據,採用不同的取樣比例(5%、10%、20%、30%和40%)、3種遺傳距離計算方法(歐氏距離、標準歐氏距離和馬氏距離)、4種繫統聚類方法(最短距離法、最長距離法、不加權類平均法和離差平方和法)和2種取樣方法(隨機取樣法和最小距離逐步取樣法)構建尾葉桉覈心種質資源庫,利用均值差異百分率(MD)、方差差異百分率(VD)、變異繫數變化率(VR)和極差符閤率(CR)作為構建覈心種質的評價指標。【結果和結論】結果錶明採用10%的抽樣比例、標準歐氏距離、最短距離繫統聚類法和最小距離逐步取樣法構建的包括25箇種源100箇傢繫的尾葉桉覈心種質最能代錶原有的種質群體。
【목적】위료쾌속、준학지종봉부적미협안Eucalyptus urophylla충질자원중감정출육충상박절수요적우이기인,초보구건료미협안핵심충질,이편간화관리,제고유전자원재미협안개량중적이용효솔。【방법】이미협안27개충원194개가계적수고、흉경、재적등17개수량성상위의거,채용불동적취양비례(5%、10%、20%、30%화40%)、3충유전거리계산방법(구씨거리、표준구씨거리화마씨거리)、4충계통취류방법(최단거리법、최장거리법、불가권류평균법화리차평방화법)화2충취양방법(수궤취양법화최소거리축보취양법)구건미협안핵심충질자원고,이용균치차이백분솔(MD)、방차차이백분솔(VD)、변이계수변화솔(VR)화겁차부합솔(CR)작위구건핵심충질적평개지표。【결과화결론】결과표명채용10%적추양비례、표준구씨거리、최단거리계통취류법화최소거리축보취양법구건적포괄25개충원100개가계적미협안핵심충질최능대표원유적충질군체。
Objective] In order to rapidly and precisely identify the excellent gene for the breeding of Eu-calyptus urophylla, the construction of core collection of E.urophylla was necessary .Establishing a core collection of E.urophylla could simplify the management and enhance the utilization of genetic resources in E.urophylla research programs.[Method] Based on 17 quantitative traits, height, diameter and vol-ume of 194 families from 27 provenances of E.urophylla, core collections were constructed using different sampling proportions ( 5%, 10%, 20%, 30%, and 40%) , 3 distances ( Euclidean distance , Standard-ized Euclidean distance , and Mahalanobis distance ) , 4 hierarchical clustering methods ( Single distance method, Complete distance method, Unweighted pair-group average method and Ward’s method), and 2 sampling strategies ( random sampling and least distance stepwise sampling ) were employed .The genetic variation of the quantitative traits among these core collections was compared by evaluating the mean difference percentage ( MD) , variance difference percentage ( VD) , changeable rate of coefficient of var-iation ( VR) and coincidence rate of range ( CR) of the traits.[Result and conclusion] The results showed that the primary core collection was constructed with 10%sampling proportion , standardized Eu-clidean distance , Single distance method , and least distance stepwise sampling , which was composed of 100 families from 27 provenances of E.urophylla and the best representative of total germplasm .