华南农业大学学报
華南農業大學學報
화남농업대학학보
JOURNAL OF SOUTH CHINA AGRICULTURAL UNIVERSITY
2014年
6期
52-57
,共6页
刘晓嘉%唐雪东%刘海广%吴林%张志东%李亚东
劉曉嘉%唐雪東%劉海廣%吳林%張誌東%李亞東
류효가%당설동%류해엄%오림%장지동%리아동
越橘%土壤改良%根系分泌物%细胞分裂素
越橘%土壤改良%根繫分泌物%細胞分裂素
월귤%토양개량%근계분비물%세포분렬소
Vaccinium vitis-idaea%soil improvement%root exudate%cytokinin
【目的】研究不同土壤改良物质对越橘Vaccinium vitis-idaea 根际土和非根际土中细胞分裂素[玉米素( Z)、玉米素核苷( ZR)和异戊烯基腺苷( iPA)]含量及年变化规律的影响.【方法】以2年生盆栽越橘为试材,应用“根际微区土壤剥落分离法”收集与根系黏着程度不同的非根际土和根际土样品,用高效液相色谱法测定土壤样品细胞分裂素含量.【结果和结论】不同根域处理的根系分泌物中,玉米秸秆配施氮肥处理( k4)的细胞分裂素含量最高,其他依次为添加玉米秸秆处理(k3)、添加草炭处理(k2)、无添加(k1,对照);不同时期越橘根际土和非根际土中细胞分裂素含量总趋势为Z>ZR>iPA,如k4处理的Z、ZR和iPA平均质量比分别为9.89、9.76和3.57 ng/g,分别比k1(对照)高出67.9%、84.2%和105.6%.越橘根系细胞分裂素含量年变化规律表现为早春时期呈上升趋势,秋末季节含量呈下降趋势,而在早春至秋末之间,根系分泌物含量较高.
【目的】研究不同土壤改良物質對越橘Vaccinium vitis-idaea 根際土和非根際土中細胞分裂素[玉米素( Z)、玉米素覈苷( ZR)和異戊烯基腺苷( iPA)]含量及年變化規律的影響.【方法】以2年生盆栽越橘為試材,應用“根際微區土壤剝落分離法”收集與根繫黏著程度不同的非根際土和根際土樣品,用高效液相色譜法測定土壤樣品細胞分裂素含量.【結果和結論】不同根域處理的根繫分泌物中,玉米秸稈配施氮肥處理( k4)的細胞分裂素含量最高,其他依次為添加玉米秸稈處理(k3)、添加草炭處理(k2)、無添加(k1,對照);不同時期越橘根際土和非根際土中細胞分裂素含量總趨勢為Z>ZR>iPA,如k4處理的Z、ZR和iPA平均質量比分彆為9.89、9.76和3.57 ng/g,分彆比k1(對照)高齣67.9%、84.2%和105.6%.越橘根繫細胞分裂素含量年變化規律錶現為早春時期呈上升趨勢,鞦末季節含量呈下降趨勢,而在早春至鞦末之間,根繫分泌物含量較高.
【목적】연구불동토양개량물질대월귤Vaccinium vitis-idaea 근제토화비근제토중세포분렬소[옥미소( Z)、옥미소핵감( ZR)화이무희기선감( iPA)]함량급년변화규률적영향.【방법】이2년생분재월귤위시재,응용“근제미구토양박락분리법”수집여근계점착정도불동적비근제토화근제토양품,용고효액상색보법측정토양양품세포분렬소함량.【결과화결론】불동근역처리적근계분비물중,옥미갈간배시담비처리( k4)적세포분렬소함량최고,기타의차위첨가옥미갈간처리(k3)、첨가초탄처리(k2)、무첨가(k1,대조);불동시기월귤근제토화비근제토중세포분렬소함량총추세위Z>ZR>iPA,여k4처리적Z、ZR화iPA평균질량비분별위9.89、9.76화3.57 ng/g,분별비k1(대조)고출67.9%、84.2%화105.6%.월귤근계세포분렬소함량년변화규률표현위조춘시기정상승추세,추말계절함량정하강추세,이재조춘지추말지간,근계분비물함량교고.
Objective] The contents and annual variations of cytokinins including zeatin (Z), zeatin ri-boside(ZR) and isopentenyl adenodine(iPA) were studied in the rhizosphere and non-rhizosphere of blueberry, Vaccinium vitis-idaea‘Northland’ after the application of different soil amelioration materials in pots.[Method] Rhizosphere and non-rhizosphere soil samples with different root adhesion degrees were collected by rhizosphere soil stripping separation method ,high performance liquid chromatography meth-ods were used to investigate cytokinin contents in soil samples. [Result and conclusion] Cytokinin con-tents of corn stalks mixed with nitrogen fertilizer(k4)were the highest, followed by the application treat-ments of corn stalks(k3), peat(k2), and no added (k1,control).The general trend of cytokinin contents in blueberry rhizosphere and non rhizosphere soil were Z >ZR>iPA in different periods, such as k4 treat-ment the Z, ZR and iPA average content was 9.89, 9.76 and 3.57 ng/g respectively, which were higher than 67.9%,84.2%and 105.6%compared with the control, respectively. The annual variations of cyto-kinins in the root zone of blueberry were traced. The contents of cytokinins increased in early spring and declined in autumn, while between in early spring and late autumn they maintained high levels.