华南农业大学学报
華南農業大學學報
화남농업대학학보
JOURNAL OF SOUTH CHINA AGRICULTURAL UNIVERSITY
2014年
6期
29-32
,共4页
陈继群%刘丽贞%陈杰忠%张海岚
陳繼群%劉麗貞%陳傑忠%張海嵐
진계군%류려정%진걸충%장해람
钙%脐橙%细胞壁酶%裂果
鈣%臍橙%細胞壁酶%裂果
개%제등%세포벽매%렬과
calcium%navel orange%cell wall enzymes%fruit cracking
【目的】揭示钙对脐橙裂果的作用机理.【方法】以抗裂果的‘纽荷尔’ Citrus sinensis cv.Newhall 和易裂果的‘朋娜’Citrus sinensis cv.Skagg’s Bonanza为试材,进行外源补钙和钙吸收抑制剂处理,在不同时期记录果实裂果率,测定果皮钙含量和纤维素酶( CX)、多聚半乳糖醛酸酶( PG)、果胶甲酯酶( PE)的酶活性.【结果和结论】抗裂的‘纽荷尔’的果皮钙含量高于易裂的‘朋娜’,同一品种正常果的果皮钙含量高于裂果;花后150 d,质量分数为2%Ca(OH)2的处理可以显著提高果皮钙含量,提高果皮硬度,降低裂果率;质量分数为2%Ca(OH)2处理与CX酶活性呈负相关关系;钙能通过影响细胞壁酶活性影响裂果,其中对‘朋娜’影响显著,对‘纽荷尔’作用不明显.
【目的】揭示鈣對臍橙裂果的作用機理.【方法】以抗裂果的‘紐荷爾’ Citrus sinensis cv.Newhall 和易裂果的‘朋娜’Citrus sinensis cv.Skagg’s Bonanza為試材,進行外源補鈣和鈣吸收抑製劑處理,在不同時期記錄果實裂果率,測定果皮鈣含量和纖維素酶( CX)、多聚半乳糖醛痠酶( PG)、果膠甲酯酶( PE)的酶活性.【結果和結論】抗裂的‘紐荷爾’的果皮鈣含量高于易裂的‘朋娜’,同一品種正常果的果皮鈣含量高于裂果;花後150 d,質量分數為2%Ca(OH)2的處理可以顯著提高果皮鈣含量,提高果皮硬度,降低裂果率;質量分數為2%Ca(OH)2處理與CX酶活性呈負相關關繫;鈣能通過影響細胞壁酶活性影響裂果,其中對‘朋娜’影響顯著,對‘紐荷爾’作用不明顯.
【목적】게시개대제등렬과적작용궤리.【방법】이항렬과적‘뉴하이’ Citrus sinensis cv.Newhall 화역렬과적‘붕나’Citrus sinensis cv.Skagg’s Bonanza위시재,진행외원보개화개흡수억제제처리,재불동시기기록과실렬과솔,측정과피개함량화섬유소매( CX)、다취반유당철산매( PG)、과효갑지매( PE)적매활성.【결과화결론】항렬적‘뉴하이’적과피개함량고우역렬적‘붕나’,동일품충정상과적과피개함량고우렬과;화후150 d,질량분수위2%Ca(OH)2적처리가이현저제고과피개함량,제고과피경도,강저렬과솔;질량분수위2%Ca(OH)2처리여CX매활성정부상관관계;개능통과영향세포벽매활성영향렬과,기중대‘붕나’영향현저,대‘뉴하이’작용불명현.
Objective] The objective of the study was to find the relationships among calcium contents , cell wall enzyme activities and fruit cracking .[Method] Two naval orange ( Citrus sinensis) cultivars,‘Skagg ’ s Bonanza ’ and ‘Newhall ’ were treated with exogenous calcium or calcium uptake inhibitor . The contents of calcium , the rate of fruit cracking and the activities of polygalacturonase ( PG) , cellulase ( CX) , and pectinmethylesterase ( PE) were investigated in different periods .[Result and conclusion]The contents of calcium in ‘Newhall’ was higher than that in ‘Skagg’s Bonanza’.The content of calci-um in the normal fruits was higher than that in cracked fruits in the same cultivar .The mass fraction 2%Ca( OH) 2 treatment increased significantly the calcium content in the pericarp and fruit firmness , reduc-ing the rate of fruit cracking 150 days after anthesis.The mass fraction 2%Ca(OH)2 treatment reduced the activities of cellulase ( CX) .Calcium affected the incidence of fruit cracking through its effect on the activities of cell wall enzymes .Its effect was significant in ‘Skagg ’ s Bonanza ’ but not in ‘Newhall ’ .