CAJ | 학술논문

目的：本研究旨在选择适合的大鼠，对比2月龄大鼠与4月龄大鼠CPR后神经功能评分及亚组评分，摸索利用不同月龄的大鼠CPR的可行性。方法通过严格监测SD大鼠制备模型过程中的心电、血压生理指标、测定各组大鼠不同时间点的神经功能缺失评分以及神经功能亚组评分、对比制备4月龄和2月龄大鼠心肺复苏模型的稳定性。结果电刺激致室颤，4月龄组大鼠的成模率为87.5％，远高于2月龄组大鼠，而死亡率无差异；在电刺激诱发CA过程中造成的血压变化，4月龄组大鼠明显低于2月龄组大鼠，存在极显著性差异（ P ＜0.01）；4月龄组大鼠与2月龄组大鼠CPR后各时间点的神经功能评分无统计学差异，而4月龄组大鼠与2月龄组大鼠的神经功能亚组评分在不同时间点，存在显著差异（P ＜0.05），4月龄大鼠与2月龄大鼠对比，心肺复苏后脑损伤的程度加重。结论4月龄大鼠更适合制备心肺复苏模型，该月龄组的大鼠制备模型成模率高，脑损伤程度重，更适合于用于心肺复苏的基础研究及治疗评价。
목적：본연구지재선택괄합적대서，대비2월령대서여4월령대서CPR후신경공능평분급아조평분，모색이용불동월령적대서CPR적가행성。방법통과엄격감측SD대서제비모형과정중적심전、혈압생리지표、측정각조대서불동시간점적신경공능결실평분이급신경공능아조평분、대비제비4월령화2월령대서심폐복소모형적은정성。결과전자격치실전，4월령조대서적성모솔위87.5％，원고우2월령조대서，이사망솔무차이；재전자격유발CA과정중조성적혈압변화，4월령조대서명현저우2월령조대서，존재겁현저성차이（ P ＜0.01）；4월령조대서여2월령조대서CPR후각시간점적신경공능평분무통계학차이，이4월령조대서여2월령조대서적신경공능아조평분재불동시간점，존재현저차이（P ＜0.05），4월령대서여2월령대서대비，심폐복소후뇌손상적정도가중。결론4월령대서경괄합제비심폐복소모형，해월령조적대서제비모형성모솔고，뇌손상정도중，경괄합우용우심폐복소적기출연구급치료평개。
Objective The purpose of this study is to select suitable ages of rats for the CPR ( cardiopulmonary resuscitation) animal model.The neurological function score and subgroups analysis are evaluated in 2 month old and 4 month old animal groups.Methods Based on the evaluation of physiological indexes including ECG, blood pressure and neurological function defect score ( NDS) and subgroup analysis, the stability of CPR rats model was compared between 2 month old and 4 month old animal groups.Results The results showed that, the model rate of the ventricular fibrillation was induced by electrical stimulation , the 4 month old group was 87.5%, significantly higher than the 2 month old group, however, there was no significant difference between the two groups in the mortality rate;For the changes of blood pressure during the process of CA( cardiac arrest) induced by electrical stimulation, the 4 month old group was significantly lower than the 2 month old group (P <0.01); for the NDS at each time point after CPR, there was no significant difference between the two groups; however, the NDS subgroup analysis at different time points showed that there were different degrees of differences between the two age groups ( P <0.05) .Comparing with the 2 month old group, the 4 month old group had a stable process during the animal model preparation, had an obvious cerebral blood hypoperfusion phenomenon and aggravation of brain injury after CPR.Conclusion The 4 month old rats are more suitable for preparation of CPR animal mode , the model rate is high, the brain injury aggravate.It is more suitable evaluation for basic research and treatment of CPR.