中国比较医学杂志
中國比較醫學雜誌
중국비교의학잡지
CHINESE JOURNAL OF COMPARATIVE MEDICINE
2014年
9期
40-43
,共4页
李晓娟%汤紫荣%李蓓%李瑞生
李曉娟%湯紫榮%李蓓%李瑞生
리효연%탕자영%리배%리서생
近交系小鼠%移植性肝癌%模型,动物
近交繫小鼠%移植性肝癌%模型,動物
근교계소서%이식성간암%모형,동물
Inbred mice%Transplanted liver cancer%Model,animal
目的：通过对比分析选择建立原位移植性肝癌模型的最佳小鼠品系。方法选取C57、C3H和BALB／c各10只小鼠分别作为模型组Ⅰ、模型组Ⅱ和模型组Ⅲ，沿腹中线开腹后将H22细胞接种到各模型组小鼠肝脏实质内。于注射后第15天剖腹探查，观察各组成瘤率，测量腹水量和肿瘤体积，并进行肿瘤病理学分析。结果三组小鼠存活率均为100％，15天后三组小鼠均产生腹水，但三组腹水量之间不具有统计学差异。模型组Ⅰ小鼠肝癌移植成功率为100％，高于模型组Ⅱ的60％和模型组Ⅲ的30％。模型组Ⅰ小鼠肝脏肿瘤全部为大块紧实灰白色病灶，其肿瘤平均体积显著大于模型组Ⅱ和模型组Ⅲ（ P＜0.05）。病理结果证实三组小鼠肝脏的灰白色病灶均为原位肝细胞癌。结论 C57小鼠是复制原位移植性肝癌模型较为理想的实验动物，为今后研究原位肝癌的发病机制提供良好的实验平台。
目的：通過對比分析選擇建立原位移植性肝癌模型的最佳小鼠品繫。方法選取C57、C3H和BALB／c各10隻小鼠分彆作為模型組Ⅰ、模型組Ⅱ和模型組Ⅲ，沿腹中線開腹後將H22細胞接種到各模型組小鼠肝髒實質內。于註射後第15天剖腹探查，觀察各組成瘤率，測量腹水量和腫瘤體積，併進行腫瘤病理學分析。結果三組小鼠存活率均為100％，15天後三組小鼠均產生腹水，但三組腹水量之間不具有統計學差異。模型組Ⅰ小鼠肝癌移植成功率為100％，高于模型組Ⅱ的60％和模型組Ⅲ的30％。模型組Ⅰ小鼠肝髒腫瘤全部為大塊緊實灰白色病竈，其腫瘤平均體積顯著大于模型組Ⅱ和模型組Ⅲ（ P＜0.05）。病理結果證實三組小鼠肝髒的灰白色病竈均為原位肝細胞癌。結論 C57小鼠是複製原位移植性肝癌模型較為理想的實驗動物，為今後研究原位肝癌的髮病機製提供良好的實驗平檯。
목적：통과대비분석선택건립원위이식성간암모형적최가소서품계。방법선취C57、C3H화BALB／c각10지소서분별작위모형조Ⅰ、모형조Ⅱ화모형조Ⅲ，연복중선개복후장H22세포접충도각모형조소서간장실질내。우주사후제15천부복탐사，관찰각조성류솔，측량복수량화종류체적，병진행종류병이학분석。결과삼조소서존활솔균위100％，15천후삼조소서균산생복수，단삼조복수량지간불구유통계학차이。모형조Ⅰ소서간암이식성공솔위100％，고우모형조Ⅱ적60％화모형조Ⅲ적30％。모형조Ⅰ소서간장종류전부위대괴긴실회백색병조，기종류평균체적현저대우모형조Ⅱ화모형조Ⅲ（ P＜0.05）。병리결과증실삼조소서간장적회백색병조균위원위간세포암。결론 C57소서시복제원위이식성간암모형교위이상적실험동물，위금후연구원위간암적발병궤제제공량호적실험평태。
Objective To select a best mouse strain for establishing an animal model of orthotopic transplanted liver cancer through comparison analysis.Methods C57,C3H and BALB/c mice( each 10) were selected as model groupⅠ,Ⅱ and Ⅲ.H22 cells were inoculated into the liver parenchyma of mice in model group along the ventral midline laparotomy.Fifteenth days after injection of laparotomy,the rate of tumor was observed,the volume of ascites and gross tumor volume were measured,and tumor pathology was analyzed.Results The survival rate of mice in three groups was 100%. After 15 days,ascites in three groups were produced,but with no significant difference among the three groups of the volume of ascites.Tumor transplantation rate in model groupⅠwas 100%,which was higher than 60%in model groupⅡand 30%in model group Ⅲ.Liver tumors in model group Ⅰ were all large compact grayish white lesions, and the average tumor volume was significantly greater than that of model group Ⅱ and Ⅲ( P <0.05 ) .Pathology results confirmed that white lesions in liver of three groups were hepatocelluar carcinoma in situ.Conclusion C57 is the better ideal experimental animal for establishing orthotopic liver caner model,and these results will provide a good experimental platform for research in pathogenesis of hepatocellular carcinoma.
