CAJ | 학술논문

目的：建立可快速检测化学物致敏性和刺激性的局部淋巴结试验（ LLNA：BrdU-ELISA）改良法，并对化妆品产品进行评价。方法3种化学物（2，4二硝基氯苯（ DNCB）、丁子香酚、己基肉桂醛）和3种化妆品产品作为受试物，雌性BALB／c小鼠连续染毒3天，测量小鼠耳缘厚度，第5天腹腔注射BrdU，第6天称耳廓重，分离颌下淋巴结称重并制取单细胞悬液，用ELISA试剂盒检测淋巴细胞增殖。结果 DNCB（1.0％）、己基肉桂醛（25％、50％）和3号粉底霜引起耳肿胀和耳廓重显著增加（P ＜0.05或P ＜0.01），可能为刺激物，其他均无刺激性；3种化学物和3号粉底霜致敏检测阳性，其他均为阴性。结论结合小鼠耳肿胀和耳廓重的LLNA：BrdU-ELISA改良法可较好地评价化学物和化妆品产品的致敏性和刺激性，有望在化妆品安全性评价中发挥重要作用。
목적：건립가쾌속검측화학물치민성화자격성적국부림파결시험（ LLNA：BrdU-ELISA）개량법，병대화장품산품진행평개。방법3충화학물（2，4이초기록분（ DNCB）、정자향분、기기육계철）화3충화장품산품작위수시물，자성BALB／c소서련속염독3천，측량소서이연후도，제5천복강주사BrdU，제6천칭이곽중，분리합하림파결칭중병제취단세포현액，용ELISA시제합검측림파세포증식。결과 DNCB（1.0％）、기기육계철（25％、50％）화3호분저상인기이종창화이곽중현저증가（P ＜0.05혹P ＜0.01），가능위자격물，기타균무자격성；3충화학물화3호분저상치민검측양성，기타균위음성。결론결합소서이종창화이곽중적LLNA：BrdU-ELISA개량법가교호지평개화학물화화장품산품적치민성화자격성，유망재화장품안전성평개중발휘중요작용。
Objective To establish a rapid modified Local lymph node assay ( LLNA) for the sensitization and irritancy potential of chemicals, and evaluate cosmetics products. Methods 3 kinds of chemicals ( 2 , 4-Dinitrochlorobenzene ( DNCB) , Eugenol and Hexyl cinnamic aldehyde) and 3 kinds of cosmetics were selected.The dorsal sides of both ears of female BALB/c mice were treated with 25μL/ear of the test solutions on 3 consecutive days, and the thickness of each ear was measured.BrdU solution was injected inter-peritoneally on day 5.On day 6, each auricle was weighed, the bilateral draining auricular lymph nodes were excised and weighed, then made into single cell suspension.BrdU was measured by ELISA using a commercial kit.Results Ear swelling and auricle weight of DNCB ( 1.0%) , Eugenol ( 25% and 50%) and number 3 cream foundation increased significantly ( P <0.05 or P <0.01), which may be considered as irritants.The others were negative.For LLNA, three chemicals and number 3 cream foundation pretended positive.Conclusion The combined LLNA/ear swelling/ear weight assay appears to be a reliable predictor of sensitization and irritancy potential, and is expected to play an important role in safety evaluation of cosmetics.