CAJ | 학술논문

目的：观察肥胖对小鼠十二指肠二价金属离子转运体（divalent metal transporter 1，DMT1）mRNA、膜铁转运蛋白（ferroportin1，FPN1）mRNA及蛋白表达的变化，探讨肥胖影响铁吸收的机制。方法 C57BL／6J小鼠随机分为正常对照组和肥胖模型组，每组6只，通过喂养高脂饲料喂养建立肥胖模型，对照组采用普通饲料饲养，实验干预期14周。建模完成后，采用实时荧光定量PCR方法检测小鼠十二指肠DMT1、FPN1 mRNA 的表达，用Western blot检测小鼠十二指肠FPN1蛋白表达。结果与对照组小鼠相比，肥胖模型组小鼠十二指肠DMT1、FPN1 mRNA表达以及FPN1蛋白表达水平降低，差异具有统计学意义（ P ＜0.05）。结论肥胖会下调机体十二指肠DMT1、FPN1的表达，导致铁吸收不良，为进一步研究肥胖引起铁缺乏机制提供理论和实验依据。
목적：관찰비반대소서십이지장이개금속리자전운체（divalent metal transporter 1，DMT1）mRNA、막철전운단백（ferroportin1，FPN1）mRNA급단백표체적변화，탐토비반영향철흡수적궤제。방법 C57BL／6J소서수궤분위정상대조조화비반모형조，매조6지，통과위양고지사료위양건립비반모형，대조조채용보통사료사양，실험간예기14주。건모완성후，채용실시형광정량PCR방법검측소서십이지장DMT1、FPN1 mRNA 적표체，용Western blot검측소서십이지장FPN1단백표체。결과여대조조소서상비，비반모형조소서십이지장DMT1、FPN1 mRNA표체이급FPN1단백표체수평강저，차이구유통계학의의（ P ＜0.05）。결론비반회하조궤체십이지장DMT1、FPN1적표체，도치철흡수불량，위진일보연구비반인기철결핍궤제제공이론화실험의거。
Objective To study the expression of divalent metal transporter 1(DMT1)and ferroportin 1(FPN1)in obese mice’ s duodenal epithelium and investigate the mechanism of the effect of obesity on iron absorption in mice. Methods C57BL/6J mice were randomly divided into control group and obesity model group, each group of 6, To establish obese mice model by having a high-fat diet and the control group were fed with a normal diet for 12 weeks.After completion of modeling, The level of DMT1 and FPN1 mRNA expression in the duodenum were measured by real-time fluorescent quantitative PCR( Real-time PCR) method, the protein expression of FPN1 was measured by Western-Blot. Results Compared with the control group, the level of DMT1、FPN1 mRNA and FPN1 protein expression in the duodenum were decreased significantly in obese mice ( P <0.05 ) .Conclusion Obesity can decrease the expression levels of DMT1、FPN1 mRNA and FPN1 protein and induce iron deficiency,in order to provide experimental and theoretical basis for studying the mechanism of iron deficiency caused by obesity further.