中国比较医学杂志
中國比較醫學雜誌
중국비교의학잡지
CHINESE JOURNAL OF COMPARATIVE MEDICINE
2014年
9期
9-13
,共5页
薛金龙%孙芳玲%刘婷婷%侯虹丽%魏仁平%向本旭%艾厚喜%王玉兰%张丽%石淑先%王文
薛金龍%孫芳玲%劉婷婷%侯虹麗%魏仁平%嚮本旭%艾厚喜%王玉蘭%張麗%石淑先%王文
설금룡%손방령%류정정%후홍려%위인평%향본욱%애후희%왕옥란%장려%석숙선%왕문
莫诺苷%Wnt7a%结肠腺瘤样息肉病蛋白%脑缺血%再灌注
莫諾苷%Wnt7a%結腸腺瘤樣息肉病蛋白%腦缺血%再灌註
막낙감%Wnt7a%결장선류양식육병단백%뇌결혈%재관주
Morroniside%Wnt7a%APC%Cerebral ischemia%Reperfusion
目的：观察莫诺苷对局灶性脑缺血再灌注大鼠Wnt7a和APC表达的影响。方法用线栓法制备大鼠局灶性脑缺血再灌注模型（ MCAO）。将50只Sprague-Dawley 大鼠随机分为假手术组，模型组，莫诺苷小、中、大剂量组（30 mg／kg、90mg／kg、270 mg／kg）。利用蛋白免疫印迹法检测Wnt7a和APC表达变化。结果造模7 d，模型组大鼠皮层Wnt7a的表达比假手术组显著升高，APC表达显著下降；与模型组相比，莫诺苷大剂量组Wnt7a表达明显升高；莫诺苷小、中、大剂量组APC的表达显著降低。结论莫诺苷可以促进缺血性脑损伤后Wnt7a表达，抑制APC表达，从而激活Wnt信号通路。
目的：觀察莫諾苷對跼竈性腦缺血再灌註大鼠Wnt7a和APC錶達的影響。方法用線栓法製備大鼠跼竈性腦缺血再灌註模型（ MCAO）。將50隻Sprague-Dawley 大鼠隨機分為假手術組，模型組，莫諾苷小、中、大劑量組（30 mg／kg、90mg／kg、270 mg／kg）。利用蛋白免疫印跡法檢測Wnt7a和APC錶達變化。結果造模7 d，模型組大鼠皮層Wnt7a的錶達比假手術組顯著升高，APC錶達顯著下降；與模型組相比，莫諾苷大劑量組Wnt7a錶達明顯升高；莫諾苷小、中、大劑量組APC的錶達顯著降低。結論莫諾苷可以促進缺血性腦損傷後Wnt7a錶達，抑製APC錶達，從而激活Wnt信號通路。
목적：관찰막낙감대국조성뇌결혈재관주대서Wnt7a화APC표체적영향。방법용선전법제비대서국조성뇌결혈재관주모형（ MCAO）。장50지Sprague-Dawley 대서수궤분위가수술조，모형조，막낙감소、중、대제량조（30 mg／kg、90mg／kg、270 mg／kg）。이용단백면역인적법검측Wnt7a화APC표체변화。결과조모7 d，모형조대서피층Wnt7a적표체비가수술조현저승고，APC표체현저하강；여모형조상비，막낙감대제량조Wnt7a표체명현승고；막낙감소、중、대제량조APC적표체현저강저。결론막낙감가이촉진결혈성뇌손상후Wnt7a표체，억제APC표체，종이격활Wnt신호통로。
Objective To investigate the effects of morroniside on the expression of Wnt7a and APC in rat cortex of focal cerebral ischemia/reperfusion.Methods After middle cerebral artery occlusion ( MCAO) model was performed SD rats were divided into groups of sham, model, and morroniside ( 30 mg /kg, 90 mg/kg,270 mg/kg) .The expression of Wnt7a and APC were evaluated by Western blot.Results Compared with the sham group, the expression of Wnt7a in rats of MCAO model was increased, and the expression of APC was decreased.Compared with model group, morroniside at the concentrations of 30 mg/kg, 90 mg/kg, 270 mg/kg decreased APC expression respectively, while 270 mg/kg morroniside-treatment obviously increased the expression of Wnt7a. Conclusion Morroniside could increase the expression of Wnt7a, and inhibit the expression of APC after ischemic injury, therefore activating the Wnt signaling pathway.
