CAJ | 학술논문

目的：探讨不同剂量Alpha-亚麻酸（ ALA）对硬脂酸培养后的HepG2细胞脂肪酸合成基因表达的影响。方法 HepG2细胞分为对照组（NC）以及含有0.5 mmol／L硬脂酸的培养液培养高脂组（HF），培养36 h后应用实时定量PCR检测脂肪酸合成关键基因SREBP1C、FAS及ACC表达；基因表达存在显著差异后分别用10％、20％、50％、70％、100％ALA替代硬脂酸培养36 h，实时定量PCR和免疫印迹法检测上述基因mRNA水平及蛋白表达。结果硬脂酸处理后HepG2细胞SREBP1C、FAS及ACC基因显著升高（P ＜0.001），ALA替代组SREBP1C基因mRNA表达水平均显著低于高脂组（ P ＜0.001），0.5 mmol／L ALA组及0.35 mmol／L ALA组FAS显著低于高脂组（ P ＜0.001），各替代组ACC基因mRNA水平与高脂组无统计学差异；替代组SREBP1C及FAS蛋白表达水平显著低于高脂组，而ACC蛋白表达量与高脂组无明显差异。结论硬脂酸促进HepG2细胞脂肪酸合成，ALA通过抑制SREBP1C及FAS基因表达来减弱脂肪酸合成。
목적：탐토불동제량Alpha-아마산（ ALA）대경지산배양후적HepG2세포지방산합성기인표체적영향。방법 HepG2세포분위대조조（NC）이급함유0.5 mmol／L경지산적배양액배양고지조（HF），배양36 h후응용실시정량PCR검측지방산합성관건기인SREBP1C、FAS급ACC표체；기인표체존재현저차이후분별용10％、20％、50％、70％、100％ALA체대경지산배양36 h，실시정량PCR화면역인적법검측상술기인mRNA수평급단백표체。결과경지산처리후HepG2세포SREBP1C、FAS급ACC기인현저승고（P ＜0.001），ALA체대조SREBP1C기인mRNA표체수평균현저저우고지조（ P ＜0.001），0.5 mmol／L ALA조급0.35 mmol／L ALA조FAS현저저우고지조（ P ＜0.001），각체대조ACC기인mRNA수평여고지조무통계학차이；체대조SREBP1C급FAS단백표체수평현저저우고지조，이ACC단백표체량여고지조무명현차이。결론경지산촉진HepG2세포지방산합성，ALA통과억제SREBP1C급FAS기인표체래감약지방산합성。
Objective To investigate the effects of different doses Alpha-Linolenic acid ( ALA ) on the expressions of fatty acid synthesis-related genes in HepG2 cells.Methods HepG2 cells were divided into two groups, normal control group (NC) and high fat group (HF) in which cells were firstly cultured for 36h in the medium contained 0.5mmol/L stearic acid.Real-time quantitative PCR was taken to detect mRNA expression of genes SREBP1C, FAS and ACC which are related to fatty synthesis.While there are significant differences in fatty synthesis, 10%, 20%, 50%, 70% and 100%ALA took the place of stearic acid, 36h later, real-time quantitative PCR and Western blotting were taken to detect mRNA and protein expression of genes related to fatty synthesis.Results SREBP1C mRNA expression of ALA substitution groups were significantly lower than the high-fat group ( P <0.001) .The FAS of 0.5 mmol/L ALA group and 0.35 mmol/L ALA group were significantly lower than the high-fat group (P <0.001).ACC genes mRNA level was not significantly different from high-fat group.SREBP1C and FAS protein expression were significantly lower than the high-fat group, but ACC showed no significant difference.Conclusions Saturated fatty acids promote hepatocyte fatty acid synthesis, ALA abates fatty acid synthesis by inhibiting FAS and SREBP1C gene expression.