CAJ | 학술논문

目的：观察齐刺胆的下合穴对实验性胆绞痛豚鼠血浆β-内啡肽（β-EP ）和八肽胆囊收缩素（ CCK-8）的影响以及镇痛效果，为临床治疗胆绞痛选择新的方法提供科学依据。方法：将60只成年雄性豚鼠随机分为正常组（10只）、模型组（10只）、药物组（20只）和齐刺组（20只）。正常组不作任何处置正常饲养，模型组、药物组、齐刺组均造模。造模成功后，药物组予双氯芬酸钠盐酸利多卡因注射液腹腔注射；齐刺组取双侧阳陵泉穴进行齐刺，每日1次，连续治疗5天。治疗结束后15 min内处死各组豚鼠，腹主动脉采取新鲜血液，匀浆、离心后分离血浆，采用放射免疫法测定β-EP和CCK-8的含量。结果：模型组与正常组比较，血浆中β-EP和CCK-8含量显著升高（ P＜0．01）；药物组和齐刺组血浆中β-EP和CCK-8含量均比模型组明显降低，有统计学意义（P＜0．01）；药物组、齐刺组比较无明显差异，无统计学意义（ P＞0．05）。结论：齐刺法刺激阳陵泉穴对实验性胆绞痛豚鼠有镇痛作用，可能与改善豚鼠血浆β-EP和CCK-8的表达水平有关。
목적：관찰제자담적하합혈대실험성담교통돈서혈장β-내배태（β-EP ）화팔태담낭수축소（ CCK-8）적영향이급진통효과，위림상치료담교통선택신적방법제공과학의거。방법：장60지성년웅성돈서수궤분위정상조（10지）、모형조（10지）、약물조（20지）화제자조（20지）。정상조불작임하처치정상사양，모형조、약물조、제자조균조모。조모성공후，약물조여쌍록분산납염산리다잡인주사액복강주사；제자조취쌍측양릉천혈진행제자，매일1차，련속치료5천。치료결속후15 min내처사각조돈서，복주동맥채취신선혈액，균장、리심후분리혈장，채용방사면역법측정β-EP화CCK-8적함량。결과：모형조여정상조비교，혈장중β-EP화CCK-8함량현저승고（ P＜0．01）；약물조화제자조혈장중β-EP화CCK-8함량균비모형조명현강저，유통계학의의（P＜0．01）；약물조、제자조비교무명현차이，무통계학의의（ P＞0．05）。결론：제자법자격양릉천혈대실험성담교통돈서유진통작용，가능여개선돈서혈장β-EP화CCK-8적표체수평유관。
Objective:To observe the effect of analgesia as well as the influence on endorphin (β-EP) and cholecystokinin-octopeptide ( CCK-8) in the plasma of the experimental biliary colic guinea, which provides a scientific basis for choosing a new way to treat biliary colic.Methods:60 adult male guinea pigs were divided into 4 groups: the normal group ( 10 guinea pigs ) , the model group ( 10 guinea pigs ) , the drug group ( 20 guinea pigs) and the triple needling group (20 guinea pigs).The normal group was fed normally without any processing,but other groups made models.In the drug group, give a intraperitoneal injection with compound di-clofenac sodium and lidocaine hydrochloride injection after making models successfully; in the triple needling group, triple needled bilateral GB34 and needled once a day continuously treating for 5 days.At the end of the treatment, sacrifice the guinea pigs in each group, and fresh blood,homogenate were taken for the examination of theβ-EP and CCK-8 contents by radiation therapy.Results:Comparison between the model group and the normal group, the contents ofβ-EP and CCK-8 in the plasma obviously increased ( P<0.01); compared with the model group, the contents of β-EP and CCK-8 in plasma both in drug group and triple needling group obviously decreased, which was statistically significant ( P<0.01); no difference could be found be-tween the drug group and the triple needling group, which had no statistical significance (P>0.05).Conclu-sion:Triple puncture at GB34 has an analgesic effect on experimental biliary colic guineas,and it is probably re-lated to the improvement of endorphin (β-EP) and cholecystokinin-octopeptide (CCK-8)’s expression levels in the plasma.