CAJ | 학술논문

目的：比较胃癌及其癌旁组织中PRNCR1含量；设计并合成针对PRNCR1基因的siRNA,转染人胃癌细胞BGC-823,观察PRNCR1含量改变对BGC-823细胞增殖活性的影响。方法 Real Time-PCR检测10对胃癌及其癌旁组织中PRNCR1含量；化学法合成针对PRNCR1的siRNAs,脂质体法转染至BGC-823细胞,转染分为细胞对照组、转染对照组、错义序列组和siRNA转染组。转染后48 h收集细胞,Real Time-PCR检测转染后细胞内PRNCR1含量变化；CCK-8检测肿瘤细胞对数生长期基因干预对于增殖活性的影响。结果 PRNCR1在胃癌组织中含量明显高于癌旁组织(P<0．05)。与细胞对照组比较,siRNA转染组细胞中PRNCR1含量明显降低,细胞增殖活性明显受到抑制(P<0．01)；而转染对照组、错义序列组与细胞对照组比较,PRNCR1含量、细胞增殖活性均无明显差异(P>0．05)。结论 PRNCR1沉默可显著抑制BGC-823细胞增殖活性。
목적：비교위암급기암방조직중PRNCR1함량；설계병합성침대PRNCR1기인적siRNA,전염인위암세포BGC-823,관찰PRNCR1함량개변대BGC-823세포증식활성적영향。방법 Real Time-PCR검측10대위암급기암방조직중PRNCR1함량；화학법합성침대PRNCR1적siRNAs,지질체법전염지BGC-823세포,전염분위세포대조조、전염대조조、착의서렬조화siRNA전염조。전염후48 h수집세포,Real Time-PCR검측전염후세포내PRNCR1함량변화；CCK-8검측종류세포대수생장기기인간예대우증식활성적영향。결과 PRNCR1재위암조직중함량명현고우암방조직(P<0．05)。여세포대조조비교,siRNA전염조세포중PRNCR1함량명현강저,세포증식활성명현수도억제(P<0．01)；이전염대조조、착의서렬조여세포대조조비교,PRNCR1함량、세포증식활성균무명현차이(P>0．05)。결론 PRNCR1침묵가현저억제BGC-823세포증식활성。
Objective To compare the PRNCR1 content in gastric cancer tissues and adjacent tissues, siRNAs for PRNCR1 gene was designed and composed to transfect the human BGC-823 cells, and the effect of PRNCR1 content change on proliferation activity in BGC-823 cells was also observed. Methods PRNCR1 contents in 10 pairs of gastric cancer tissues and adjacent tissues were collected by Real Time-PCR ( RT-PCR) method; chemically synthetic siRNAs for PRNCR1 were transfected into BGC-823 cells by liposomes method, and then were divided into cell control group, transfection control group, noncoding group and siRNA transfection group. Cells were collected 48 h after transfection, and PRNCR1 content change was detected by RT-PCR method;CCK-8 method was used to detect the effect of genetic in-tervention on proliferative activity of tumor cells during the logarithmic growth phase. Results PRNCR1 content in the tumor tissues was significantly higher than that in the adjacent tissues ( P<0. 05 ); compared with those in cell control group, PRNCR1 level in siRNA transfection group was significantly reduced ,and proliferation activity of cells was signifi-cantly inhibited (P<0. 01);compared with those in cell control group, there were no significant differences in the PRN-CR1 content and proliferation activity of cells in transfection control group or noncoding group (P>0. 05). Conclusion PRNCR1 gene silencing can effectively inhibit proliferation activity of BGC-823 cells.