CAJ | 학술논문

目的：通过分离培养锁骨颅骨发育不良( CCD)患者的牙髓细胞，观察其牙髓细胞的生物学特性及其超微结构。方法临床收集1例CCD患者的下恒牙，分离培养牙髓细胞，检测细胞生长曲线，流式细胞仪检测细胞周期，透射电镜观察细胞超微结构，并与正常牙髓组织比较。结果成功分离培养CCD患者牙髓细胞，与正常人相比， CCD患者的牙髓细胞生长趋势较为缓慢，处于G1期的细胞比例显著增高( P<0．01)，处于S期的细胞比例显著降低(P<0．01)。透射电镜结果显示CCD患者的牙髓细胞胞质内呈现大小不等的板层状小体，粗面内质网扩张。结论本研究成功分离培养了CCD患者恒牙的牙髓细胞，其生长特性及超微结构均发生了一定程度的改变。
목적：통과분리배양쇄골로골발육불량( CCD)환자적아수세포，관찰기아수세포적생물학특성급기초미결구。방법림상수집1례CCD환자적하항아，분리배양아수세포，검측세포생장곡선，류식세포의검측세포주기，투사전경관찰세포초미결구，병여정상아수조직비교。결과성공분리배양CCD환자아수세포，여정상인상비， CCD환자적아수세포생장추세교위완만，처우G1기적세포비례현저증고( P<0．01)，처우S기적세포비례현저강저(P<0．01)。투사전경결과현시CCD환자적아수세포포질내정현대소불등적판층상소체，조면내질망확장。결론본연구성공분리배양료CCD환자항아적아수세포，기생장특성급초미결구균발생료일정정도적개변。
Objective To observe the biological characteristics and ultrastructure during the dental pulp cells of patients with cleidocranial dysplasia ( CCD) by dental pulp cells of separation culture. Methods The dental pulp cells in lower permanent teeth of one CCD patient were isolated and cultured successfully, the growth curve of cells was detec-ted, and cell cycle was detected by flow cytometer, and ultrastructure was detected by transmission electron microscopy, and then all the results the were compared with those in normal pulp tissue. Results Compared with those in normal pulp tissue, in successfully isolated and cultured dental pulp cells in vitro of CCD patient, CCD dental pulp cells grew slower, and the ratio during G1 phase was significantly higher ( P<0. 01 ) , but the ratio during S phase was significantly lower (P<0. 01). Transmission electron microscopy showed that rough endoplasmic reticulum dilatation and many different sizes of lamellar bodies in CCD dental pulp cells. Conclusion The dental pulp cells in permanent teeth can be successfully iso-lated and cultured, and the biological characteristics and ultrastructure can be changed to a certain degree.