CAJ | 학술논문

目的：探讨长时间吸入低浓度异氟烷对新生大鼠皮质星形胶质细胞的形态及幼鼠空间学习和记忆能力的影响。方法43只新生Wisrar大鼠随机分成两组，I组吸入1.1％的异氟烷6 h，C组吸入压缩空气和氧气的混合气体6 h，两组均维持吸入氧浓度为30％。在吸入麻醉后12、24、72、168 h两组分别取5只幼鼠，灌注取脑后用免疫荧光法标定星形胶质细胞，用Image J软件分析星形胶质细胞形态学的改变。在吸入异氟烷后6周，记录剩余幼鼠的存活率并将剩下的动物进行Morris水迷宫实验测试其空间学习和记忆能力。结果与C组相比，I组12 h和24 h激活星形胶质细胞的数目减少（P＜0.05），12 h时抑制状态的星形胶质细胞的数目明显上升（P＜0.05），其余时间点两组之间星形胶质细胞数目差异无统计学意义。两组幼鼠定位航行实验和空间探索实验结果差异无统计学意义。结论长时间吸入低浓度异氟烷会导致星形胶质细胞形态的暂时性改变，但是对于幼鼠成年后的认知功能无明显影响。
목적：탐토장시간흡입저농도이불완대신생대서피질성형효질세포적형태급유서공간학습화기억능력적영향。방법43지신생Wisrar대서수궤분성량조，I조흡입1.1％적이불완6 h，C조흡입압축공기화양기적혼합기체6 h，량조균유지흡입양농도위30％。재흡입마취후12、24、72、168 h량조분별취5지유서，관주취뇌후용면역형광법표정성형효질세포，용Image J연건분석성형효질세포형태학적개변。재흡입이불완후6주，기록잉여유서적존활솔병장잉하적동물진행Morris수미궁실험측시기공간학습화기억능력。결과여C조상비，I조12 h화24 h격활성형효질세포적수목감소（P＜0.05），12 h시억제상태적성형효질세포적수목명현상승（P＜0.05），기여시간점량조지간성형효질세포수목차이무통계학의의。량조유서정위항행실험화공간탐색실험결과차이무통계학의의。결론장시간흡입저농도이불완회도치성형효질세포형태적잠시성개변，단시대우유서성년후적인지공능무명현영향。
Objective To evaluate the effects of prolonged exposure to isoflurane on the morphology of the astro-cytes in the cortex and the memory and learning ability of neonate rats.Methods Seven-days-old littermates ( n=43) of Wistar rats were randomly assigned to isoflurane group (Group I) and control group (Group C).Both groups expe-rienced either 6-hour exposure to 1.1%isoflurane or air with 30%oxygen.Five rats in each group were euthanized 12 h, 24 h, 3 d and 7 d after exposure.Brain sections were stained with glial fibrillary acidic protein markers.Astrocytes was classified and quantified in the cortex according to the morphology by the Image J programme.Spatial memory and learning ability were examined in the Morris Water Maze.Results The activated astrocytes were significantly reduced in subjects exposed to isoflurane at 12 h and 24 h in the cortex region (P<0.05), while the depressed astrocytes were increase at 12 h ( P<0.05 ) .There was no statistical difference between the 2 groups according to the spatial reference memory and learning ability.Conclusion Prolonged isoflurane exposure of neonate rats evokes an transiently decrease of activation of the astrocytes and increase of depressed astrocytes, however does not impair the juvenile memory and learning ability.