CAJ | 학술논문

目的：探讨全反式维甲酸（ ATRA）对淀粉样蛋白（ Aβ）42诱导的星形胶质细胞凋亡的影响及其作用机制。方法原代培养星形胶质细胞，分为3组，正常组加入200μL正常的DMEM／F12培养基，对照组加入200μL不含ATRA而含有2μg／mL Aβ42培养基，实验组分别加入200μL含有0.01、0.1、1、10μmol／L ATRA且同时含有2μg／mL Aβ42培养基。 MTT法检测细胞存活率，qRT-PCR、Western blot法分别检测载脂蛋白（ Apo） E和BACE1的mRNA、蛋白表达变化。结果各实验组星形胶质细胞凋亡作用不同，其中实验组0.1、1μmol／L的星形胶质细胞凋亡率较低，差异有统计学意义（ P＜0.05）。与对照组比较，实验组0.1、1μmol／L中ApoE的mR-NA、蛋白表达上调，差异有统计学意义（P＜0.01），BACE1 mRNA、蛋白表达无明显变化。结论 ATRA可以上调ApoE表达，抑制Aβ42诱导的星形胶质细胞凋亡。低浓度ATRA对星形胶质细胞有保护作用。
목적：탐토전반식유갑산（ ATRA）대정분양단백（ Aβ）42유도적성형효질세포조망적영향급기작용궤제。방법원대배양성형효질세포，분위3조，정상조가입200μL정상적DMEM／F12배양기，대조조가입200μL불함ATRA이함유2μg／mL Aβ42배양기，실험조분별가입200μL함유0.01、0.1、1、10μmol／L ATRA차동시함유2μg／mL Aβ42배양기。 MTT법검측세포존활솔，qRT-PCR、Western blot법분별검측재지단백（ Apo） E화BACE1적mRNA、단백표체변화。결과각실험조성형효질세포조망작용불동，기중실험조0.1、1μmol／L적성형효질세포조망솔교저，차이유통계학의의（ P＜0.05）。여대조조비교，실험조0.1、1μmol／L중ApoE적mR-NA、단백표체상조，차이유통계학의의（P＜0.01），BACE1 mRNA、단백표체무명현변화。결론 ATRA가이상조ApoE표체，억제Aβ42유도적성형효질세포조망。저농도ATRA대성형효질세포유보호작용。
Objective To investigate the inhibitive effects of all-trans retinoic acid( ATRA) on amyloid β-peptide 42 ( Aβ42) -induced apoptosis of astrocytes.Methods The MTT assay was applied for detection of cell surviv-al, while qRT-PCR and Western blot were applied for assessment of ApoE and BACE1 expression.Astrocytes were cul-tured with different concentrations of ATRA for induction of apoptosis, while DMEM/F12 medium alone was used as con-trol.Results The effects of the different concentrations of ATRA on protecting against the Aβ42-induced apoptosis of astrocytes were different, as ATRA in 0.1 and 1 μmol/L significantly inhibited the apoptosis induced by Aβ42 ( P<0.05).Compared with control group, the expression of ApoE was significantly up-regulated by ATRA in 0.1 and 1μmol/L (P<0.01), while there was no significant difference in the expression of BACE1.Conclusion ATRA can up-regulate the expression of ApoE, prohibit the astrocytes apoptosis induced by Aβ42.Low concentration ATRA have protective effect on astrocytes.