CAJ | 학술논문

目的：探讨胆汁酸对人肝内胆管瘢痕成纤维细胞生长的影响。方法原代培养人肝内胆管瘢痕组织成纤维细胞。倒置显微镜下观察细胞生长的形态变化，采用噻唑蓝（ MTT ）法、细胞计数细胞和划痕实验检测胆汁酸对瘢痕成纤维细胞生长的影响。结果（1）成功原代培养人肝内胆管瘢痕成纤维细胞。（2） MTT显示在72 h，浓度0.5、1.0、2.5、5μmol／L的胆酸（CA）和脱氧胆酸（DCA）促进瘢痕成纤维细胞活力（P＜0.05），其他胆汁酸对瘢痕成纤维细胞的生长无明显影响（P＞0.05）；浓度在10、20、50、100、250、500μmol／L，游离胆汁酸和结合胆汁酸抑制瘢痕成纤维细胞的活力（P＜0.05）。同时，单独使用CA（2．5μmol／L）或DCA（5μmol／L）或联合使用CA （2．5μmol／L）和DCA（5μmol／L），与未添加胆汁酸比较，细胞数量、活力明显增加，愈合距离明显缩小（P＜0.05）；联合使用与单独使用比较，细胞数量、活力和愈合距离差异无统计学意义（P＞0.05）。结论游离胆汁酸（ CA、DCA）在肝内胆管瘢痕的形成过程中可能扮演重要角色。
목적：탐토담즙산대인간내담관반흔성섬유세포생장적영향。방법원대배양인간내담관반흔조직성섬유세포。도치현미경하관찰세포생장적형태변화，채용새서람（ MTT ）법、세포계수세포화화흔실험검측담즙산대반흔성섬유세포생장적영향。결과（1）성공원대배양인간내담관반흔성섬유세포。（2） MTT현시재72 h，농도0.5、1.0、2.5、5μmol／L적담산（CA）화탈양담산（DCA）촉진반흔성섬유세포활력（P＜0.05），기타담즙산대반흔성섬유세포적생장무명현영향（P＞0.05）；농도재10、20、50、100、250、500μmol／L，유리담즙산화결합담즙산억제반흔성섬유세포적활력（P＜0.05）。동시，단독사용CA（2．5μmol／L）혹DCA（5μmol／L）혹연합사용CA （2．5μmol／L）화DCA（5μmol／L），여미첨가담즙산비교，세포수량、활력명현증가，유합거리명현축소（P＜0.05）；연합사용여단독사용비교，세포수량、활력화유합거리차이무통계학의의（P＞0.05）。결론유리담즙산（ CA、DCA）재간내담관반흔적형성과정중가능분연중요각색。
Objective To investigate the effects of bile acid on the growth of scar fibroblasts in human intrahepat-ic bile ducts.Methods Primary scar fibroblasts from human intrahepatic bile ducts were co-culture with bile acid.Mor-phological changes were observed under microscope.The growth of scar fibroblast was tested by MTT method, cell count and wound-healing test.Results The primary scar fibroblasts of intrahepatic bile ducts were successfully cultured.De-termined by MTT, significant promotion on the vitality of scar fibroblasts was observed in CA and DCA in the concentra-tions of 0.5, 1.0, 2.5, 5 μmol/L (P<0.05), while the other bile acids had no effect on the growth of scar fibroblasts (P>0.05).However, in concentration of 10, 20, 50, 100, 250, 500 μmol/L, the free bile acids and conjugated bile acids significantly inhibited the growth of scar fibroblasts (P<0.05).The count and vitality of the scar fibroblasts with the CA plus DCA, CA alone or DCA alone were significantly higher than the fibroblasts with CA plus DCA, CA alone and DCA alone (P>0.05).Conclusion Cholic acid and deoxycholic acid play important role in the formation of scar in the intrahepatic bile ducts.