CAJ | 학술논문

目的探讨孕妇血清IgG亚类检测对新生儿溶血疾病(HDN)早期诊断的临床价值.方法选取我院于2012年9月至2013年9月收治62例血型不合、O型RhD(+)孕妇及其新生儿血样作为研究对象,采用试管法测定血型的IgG抗体特异性,并采用EL ISA法进行血清IgG亚类的定量检测.结果 HDN(+)新生儿及其母亲的IgG1水平明显高于HDN(-)新生儿及其母亲,差异有统计学意义(P＜0.05);但IgG2、IgG3及IgG4水平比较差异无统计学意义(P＞0.05).结论对孕妇血清IgG亚类的监测,能准确地预报出HDN的发生,对HDN的早期临床诊断具有重要的意义.
목적 탐토잉부혈청IgG아류검측대신생인용혈질병(HDN)조기진단적림상개치.방법 선취아원우2012년9월지2013년9월수치62례혈형불합、O형RhD(+)잉부급기신생인혈양작위연구대상,채용시관법측정혈형적IgG항체특이성,병채용EL ISA법진행혈청IgG아류적정량검측.결과 HDN(+)신생인급기모친적IgG1수평명현고우HDN(-)신생인급기모친,차이유통계학의의(P＜0.05);단IgG2、IgG3급IgG4수평비교차이무통계학의의(P＞0.05).결론 대잉부혈청IgG아류적감측,능준학지예보출HDN적발생,대HDN적조기림상진단구유중요적의의.
Objective To explore the diagnostic value of serum IgG subclass test of pregnant women in hemolytic disease of newborn (HDN).Methods 62 cases of patients with blood group incompatibility,blood type O RhD(+) and newborn blood samples were selected as the research objects.Test tube method was used to measure the immunoglobulin G (IgG) antibody specificity,the ELISA method was used to measure the serum IgG subclass.Results The levels of IgG1 in HDN(+) neonatal and their mothers were higher than those in HDN(-) neonatal and their mothers (P ＜ 0.05); there was no significant difference found in the levels of IgG2,IgG3 and IgG4 (P ＞ 0.05).Conclusion The test to serum IgG subclass of pregnant women can precisely forecast HDN incidence,and it has important significance in clinical diagnosis.