CAJ | 학술논문

目的：探讨大鼠基础痛阈与创伤性应激反应强度的相关性。方法将100只健康成年SD大鼠随机均分为实验组和对照组各50只。实验组测定基础血压、心率和甩尾反应潜伏期（ TFL）；30 min后采用截肢法建立创伤模型，建模30 min后检测平均血压、心率、TFL及血清去甲肾上腺素（ NE）和丙二醛（ MDA）水平、超氧化物歧化酶（ SOD）活性。对照组不作任何创伤，监测项目及监测时点与实验组相同。采用Pearson相关分析法分析两组基础痛阈（建模前TFL）与建模前后平均动脉压和心率的差值，建模后血清NE和MDA水平、SOD活性的关系。结果两组基础痛阈无显著差异，对照组截肢前后平均动脉压及心率无统计学差异，截肢后实验组平均血压和心率差高于截肢前及对照组（P均＜0．05）。实验组截肢后血清NE、MDA水平及SOD活性高于对照组（P＜0．05）。实验组基础痛阈与截肢前后平均动脉压和心率差值，建模后血清NE、MDA水平量及SOD活性均呈负性相关。结论大鼠基础痛阈与截肢创伤引起的应激反应强度呈负性相关，即基础痛阈越低，创伤性应激反应强度越大。
목적：탐토대서기출통역여창상성응격반응강도적상관성。방법장100지건강성년SD대서수궤균분위실험조화대조조각50지。실험조측정기출혈압、심솔화솔미반응잠복기（ TFL）；30 min후채용절지법건립창상모형，건모30 min후검측평균혈압、심솔、TFL급혈청거갑신상선소（ NE）화병이철（ MDA）수평、초양화물기화매（ SOD）활성。대조조불작임하창상，감측항목급감측시점여실험조상동。채용Pearson상관분석법분석량조기출통역（건모전TFL）여건모전후평균동맥압화심솔적차치，건모후혈청NE화MDA수평、SOD활성적관계。결과량조기출통역무현저차이，대조조절지전후평균동맥압급심솔무통계학차이，절지후실험조평균혈압화심솔차고우절지전급대조조（P균＜0．05）。실험조절지후혈청NE、MDA수평급SOD활성고우대조조（P＜0．05）。실험조기출통역여절지전후평균동맥압화심솔차치，건모후혈청NE、MDA수평량급SOD활성균정부성상관。결론대서기출통역여절지창상인기적응격반응강도정부성상관，즉기출통역월저，창상성응격반응강도월대。
Objective To investigate the correlation between pain threshold and the intensity of traumatic stress reac-tion in rats.Methods A total of 100 adult SD rats were randomly divided into two groups (50 rats in each group), which were experimental group and control group.Rats in experimental group were measured blood pressure, heart rate and tail-flick latency ( TFL) , then were adopted amputation wound model by the method of amputate after 30 minutes.Thirty mi-nutes later, blood pressure and heart rate were measured again;TFL was tested;serum norepinephrine ( NE) , malondial-dehyde ( MDA) level and superoxide dismutase ( SOD) activity were detected.Rats in control group did not adopt wound model and were tested the same experimental subjects at the same time as experimental group.The correlation between pain threshold and average blood pressure, heart rate changes, correlation between serum NE and MDA content, SOD activity were analyzed by method of Pearson correlation test.Results The difference of pain threshold was not statistically signifi-cant between the two groups.The difference of average blood pressure and heart rate in control group was not significant. The average blood pressure and heart rate after amputation in experimental group were higher than amputation before as well as that in control group (P<0.05).Serum NE, MDA content and SOD activity in experimental group were higher than that in control group (P<0.05).In experimental rats group, pain threshold was negatively correlated with the difference of average blood pressure and heart rate changes before and after amputation.In experimental rats group, serum NE content was negatively correlated with MDA content and SOD activity.Conclusion The pain threshold was negatively correlated with the intensity of traumatic stress reaction, which means the lower pain threshold, the more intensity of traumatic stress reaction.