山东医药
山東醫藥
산동의약
SHANDONG MEDICAL JOURNAL
2014年
38期
11-14
,共4页
巨噬细胞移动抑制因子%诱导型一氧化氮合酶%胎膜早破%绒毛膜羊膜炎
巨噬細胞移動抑製因子%誘導型一氧化氮閤酶%胎膜早破%絨毛膜羊膜炎
거서세포이동억제인자%유도형일양화담합매%태막조파%융모막양막염
macrophage migration inhibition factor%inducible nitric oxide synthase%premature rupture of fetal mem-brane%chorioamnionitis
目的：探讨巨噬细胞移动抑制因子（ MIF）与诱导型一氧化氮合酶（ iNOS）在胎膜早破（ PROM）发生、发展中的作用。方法研究对象为90例剖宫产的产妇；其中早产胎膜早破（pPROM）30例（pPROM组），足月胎膜早破（ tPROM）30例（ tPROM组），正常足月妊娠30例（正常组）。三组均于剖宫产术中胎盘娩出后于距离胎膜破口＞2 cm处取1 cm ×1 cm的胎膜组织制作切片。采用HE染色方法检测绒毛膜羊膜炎（中性粒细胞中、重度浸润）发生情况；采用免疫组化SP法检测MIF、iNOS表达；分析三组及绒毛膜羊膜炎、非绒毛膜羊膜炎产妇MIF、iNOS表达情况。结果三组共检出绒毛膜羊膜炎27例；MIF 和 iNOS 主要表达于羊膜上皮细胞的胞质。 pPROM 组和tPROM组MIF表达强度明显高于对照组（P＜0．01）；pPROM组iNOS表达强度明显高于对照组（P＜0．01）；绒毛膜羊膜炎产妇胎膜中MIF和iNOS表达强度明显高于非绒毛膜羊膜炎产妇（P＜0．05）。结论 PROM产妇胎膜组织中MIF、iNOS表达上调。 MIF、iNOS高表达可促进胎膜早破及绒毛膜羊膜炎的发生。
目的：探討巨噬細胞移動抑製因子（ MIF）與誘導型一氧化氮閤酶（ iNOS）在胎膜早破（ PROM）髮生、髮展中的作用。方法研究對象為90例剖宮產的產婦；其中早產胎膜早破（pPROM）30例（pPROM組），足月胎膜早破（ tPROM）30例（ tPROM組），正常足月妊娠30例（正常組）。三組均于剖宮產術中胎盤娩齣後于距離胎膜破口＞2 cm處取1 cm ×1 cm的胎膜組織製作切片。採用HE染色方法檢測絨毛膜羊膜炎（中性粒細胞中、重度浸潤）髮生情況；採用免疫組化SP法檢測MIF、iNOS錶達；分析三組及絨毛膜羊膜炎、非絨毛膜羊膜炎產婦MIF、iNOS錶達情況。結果三組共檢齣絨毛膜羊膜炎27例；MIF 和 iNOS 主要錶達于羊膜上皮細胞的胞質。 pPROM 組和tPROM組MIF錶達彊度明顯高于對照組（P＜0．01）；pPROM組iNOS錶達彊度明顯高于對照組（P＜0．01）；絨毛膜羊膜炎產婦胎膜中MIF和iNOS錶達彊度明顯高于非絨毛膜羊膜炎產婦（P＜0．05）。結論 PROM產婦胎膜組織中MIF、iNOS錶達上調。 MIF、iNOS高錶達可促進胎膜早破及絨毛膜羊膜炎的髮生。
목적：탐토거서세포이동억제인자（ MIF）여유도형일양화담합매（ iNOS）재태막조파（ PROM）발생、발전중적작용。방법연구대상위90례부궁산적산부；기중조산태막조파（pPROM）30례（pPROM조），족월태막조파（ tPROM）30례（ tPROM조），정상족월임신30례（정상조）。삼조균우부궁산술중태반면출후우거리태막파구＞2 cm처취1 cm ×1 cm적태막조직제작절편。채용HE염색방법검측융모막양막염（중성립세포중、중도침윤）발생정황；채용면역조화SP법검측MIF、iNOS표체；분석삼조급융모막양막염、비융모막양막염산부MIF、iNOS표체정황。결과삼조공검출융모막양막염27례；MIF 화 iNOS 주요표체우양막상피세포적포질。 pPROM 조화tPROM조MIF표체강도명현고우대조조（P＜0．01）；pPROM조iNOS표체강도명현고우대조조（P＜0．01）；융모막양막염산부태막중MIF화iNOS표체강도명현고우비융모막양막염산부（P＜0．05）。결론 PROM산부태막조직중MIF、iNOS표체상조。 MIF、iNOS고표체가촉진태막조파급융모막양막염적발생。
Objective To study the effect of macrophage migration inhibition factor( MIF) and inducible nitric oxide synthase (iNOS) in the development of premature rupture of fetal membranes(PROM).Methods A total of 90 parturient women who had delivered by cesarean section before labor were selected in the study and were divided into 3 groups, which were preterm premature rupture of the membranes group ( pPROM group, 30 cases) , term premature rupture of the mem-branes group(tPROM group, 30 cases) and full-term cesarean section delivery group (control group, 30 cases).The membranes were collected from the >2 cm point of rupture of membranes about 1 cm ×1 cm in cesarean section after preg-nancy termination with placenta deliveried and made into paraffin sections.Chorioamnionitis was identified by using HE staining to determine the neutrophilic granulocyte infiltration.The distributions and expression levels of MIF and iNOS in fetal membranes were detected by immuno histochemical method.The expression of MIF and iNOS in the three groups were analyzed.Results A total of 27 cases chorioamnionitises were found.MIF and iNOS located in cytoplasm of amnio-endo-thelial cells.The expression of MIF in fetal membrane cells in pPROM group and tPROM group were higher than that in control group(P<0.01).The expression of iNOS in pPROM group was higher than that in control group (P<0.01).The expression levels of MIF and iNOS in chorioamnionitis puerperas were significantly higher than those in non-chorioamnionitis puerperas(P<0.05).Conclusions The expression of MIF and iNOS increase in fetal membrane of RPOM puerperas. The overexpression of MIF and iNOS may promote the development of PROM and chorioamnionitis.
