CAJ | 학술논문

目的：探讨国产地西他滨（ DEC）对小鼠淋巴瘤／白血病细胞EL4的体外细胞毒作用及对共刺激分子CD80表达的影响。方法小鼠淋巴瘤／白血病EL4细胞系（ EL4细胞）分别用进口地西他滨（ DAC）和DEC（终浓度均为0．25μM）连续体外处理3 d。采用瑞氏吉姆萨染色法观察细胞形态变化，流式细胞仪检测细胞表面共刺激分子CD80表达，CCK8法测细胞增殖抑制情况。结果 DAC和DEC均可引起EL4细胞明显的形态改变，二者作用无明显差异；DAC和DECF干预后EL4细胞CD80表达均升高，细胞增殖受抑制；二者的作用无显著差异。结论 DAC和DEC体外致EL4细胞形态改变、增殖抑制及刺激CD80表达的能力无明显差异；提示二者体内抗肿瘤作用及诱导特异性抗肿瘤免疫应答能力相近。
목적：탐토국산지서타빈（ DEC）대소서림파류／백혈병세포EL4적체외세포독작용급대공자격분자CD80표체적영향。방법소서림파류／백혈병EL4세포계（ EL4세포）분별용진구지서타빈（ DAC）화DEC（종농도균위0．25μM）련속체외처리3 d。채용서씨길모살염색법관찰세포형태변화，류식세포의검측세포표면공자격분자CD80표체，CCK8법측세포증식억제정황。결과 DAC화DEC균가인기EL4세포명현적형태개변，이자작용무명현차이；DAC화DECF간예후EL4세포CD80표체균승고，세포증식수억제；이자적작용무현저차이。결론 DAC화DEC체외치EL4세포형태개변、증식억제급자격CD80표체적능력무명현차이；제시이자체내항종류작용급유도특이성항종류면역응답능력상근。
Objective To compare the influence of cytotoxicity and CD80 expression in EL4 cells, following in vitro treatment with decitabine ( DEC) manufactured internal.Methods EL4 cell line was treated with Dacogen ( DAC, manu-factured by Xian Janssen Pharmaceutical Ltd) or DEC (final concentration 0.25μmol/L) in vitro for 3 days.Morphology was analysised by method of Wright-Gimesa staining.CD80 expression was detected by method of flow cytometry.Cell pro-liferation was detected by method of CCK-8 kit.Results DAC and DEC treatment resulted in similar morphological chan-ges in EL4 cells.Moreover, effects of CD80 expression and inhibited proliferation induced by both DAC and DEC in EL4 cells were no significant differences (P>0.05).Conclusions There was no significant difference between DAC and DEC in the ability of change cellular morphology, inhibit proliferation and stimulate CD80 expression in mouse-lymphoma/leuke-mia cell EL4 cultured in vitro, which indicates the effects of the two drugs were similarity in both cytotoxic effect on tumor cells and stimulation of anti-tumor immune response when administered in vivo.