CAJ | 학술논문

目的：探讨肝组织内雄激素受体（AR）和雌激素受体-α（ERα）含量对慢性乙型肝炎（CHB）患者血清 HBV DNA载量、HBsAg水平和肝组织内 HBsAg、HBcAg表达水平影响。方法135例经肝活组织检查的CHB患者入选本研究，其中 HBeAg阳性80例，HBeAg阴性55例。肝组织内AR mRNA和ERαmRNA采用反转录定量PCR检测。结果 HBeAg阳性患者，血清HBV DNA载量与肝组织内AR mRNA和ERαmRNA含量均无显著相关性（r＝0．132，P＝0．241和r＝0．130，P＝0．249），血清 HBsAg 水平与肝组织内 AR mRNA 和 ERαmRNA 含量均呈显著正相关（r＝0．355，P＝0．001和r＝0．246，P＝0．028）；肝组织内 HBcAg 表达水平与肝组织内AR mRNA和 ERαmRNA 含量均呈显著正相关（rs＝0．438，P＝0．000和rs＝0．352，P＝0．002），肝组织内 HBsAg 表达水平与肝组织内AR mRNA和ERαmRNA含量无显著相关性（rs＝0．112，P＝0．333和rs＝0．024，P＝0．836）。HBeAg 阴性患者，血清 HBV DNA载量与肝组织内AR mRNA和ERαmRNA含量均无显著相关性（r＝-0．256，P＝0．061和r＝-0．121，P＝0．385），血清HBsAg水平与肝组织内AR mRNA和ERαmRNA含量均无显著相关性（r＝0．130，P＝0．348和r＝0．165，P＝0．234）；肝组织内 HBcAg表达水平与肝组织内AR mRNA和ERαmRNA含量均无显著相关性（rs＝-0．053，P＝0．701和rs＝-0．203，P＝0．137），肝组织内 HBsAg表达水平与肝组织内AR mRNA和ERαmRNA含量无显著相关性（rs＝0．159， P＝0．247和rs＝0．192，P＝0．160）。多因素方差分析和有序Logistic 回归分析的结果显示，肝组织内AR mRNA 为影响HBeAg阳性患者血清 HBsAg水平和肝组织内 HBcAg表达水平的独立因素。结论肝组织内AR表达水平是影响CHB患者 HBV抗原表达的一个独立因素。
목적：탐토간조직내웅격소수체（AR）화자격소수체-α（ERα）함량대만성을형간염（CHB）환자혈청 HBV DNA재량、HBsAg수평화간조직내 HBsAg、HBcAg표체수평영향。방법135례경간활조직검사적CHB환자입선본연구，기중 HBeAg양성80례，HBeAg음성55례。간조직내AR mRNA화ERαmRNA채용반전록정량PCR검측。결과 HBeAg양성환자，혈청HBV DNA재량여간조직내AR mRNA화ERαmRNA함량균무현저상관성（r＝0．132，P＝0．241화r＝0．130，P＝0．249），혈청 HBsAg 수평여간조직내 AR mRNA 화 ERαmRNA 함량균정현저정상관（r＝0．355，P＝0．001화r＝0．246，P＝0．028）；간조직내 HBcAg 표체수평여간조직내AR mRNA화 ERαmRNA 함량균정현저정상관（rs＝0．438，P＝0．000화rs＝0．352，P＝0．002），간조직내 HBsAg 표체수평여간조직내AR mRNA화ERαmRNA함량무현저상관성（rs＝0．112，P＝0．333화rs＝0．024，P＝0．836）。HBeAg 음성환자，혈청 HBV DNA재량여간조직내AR mRNA화ERαmRNA함량균무현저상관성（r＝-0．256，P＝0．061화r＝-0．121，P＝0．385），혈청HBsAg수평여간조직내AR mRNA화ERαmRNA함량균무현저상관성（r＝0．130，P＝0．348화r＝0．165，P＝0．234）；간조직내 HBcAg표체수평여간조직내AR mRNA화ERαmRNA함량균무현저상관성（rs＝-0．053，P＝0．701화rs＝-0．203，P＝0．137），간조직내 HBsAg표체수평여간조직내AR mRNA화ERαmRNA함량무현저상관성（rs＝0．159， P＝0．247화rs＝0．192，P＝0．160）。다인소방차분석화유서Logistic 회귀분석적결과현시，간조직내AR mRNA 위영향HBeAg양성환자혈청 HBsAg수평화간조직내 HBcAg표체수평적독립인소。결론간조직내AR표체수평시영향CHB환자 HBV항원표체적일개독립인소。
Objective To inquire into the influences of androgen receptor (AR)and estrogen-α(ERα)in liver tissue in patients with chronic hepatitis B (CHB)on HBV DNA and HBsAg level in serum,and HBsAg and HBcAg expression level in liver tissue. Methods 135 patients with CHB were enrolled in present study,in which 80 patients and 55 patients respectively were HBeAg-positive and HBeAg-negative. AR mRNA and ERαmRNA in liver tissue were assayed by reverse quantitative PCR. Results In HBeAg-positive patients,serum HBV DNA level was not correlated significantly with both AR mRNA and ERαmRNA level in liver tissue(r= 0.132,P= 0.241 和r= 0.130,P= 0.249),and serum HBsAg level was positively correlated significantly with both AR mRNA and ERαmRNA level in liver tissue(r= 0.355,P= 0.001 和r=0.246,P= 0.028);HBcAg expression level in liver tissue was positively correlated significantly with both AR mRNA and ERαmRNA level in liver tissue(rs= 0.438,P= 0.000 和rs= 0.352,P= 0.002),,and HBsAg expression level in liver tissue was not correlated significantly with both AR mRNA and ERαmRNA level in liver tissue(rs= 0.112,P= 0.333 和rs= 0.024,P= 0.836). In HBeAg-negative patients,serum HBV DNA level was not correlated significantly with both AR mRNA and ERαmRNA level in liver tissue (r= -0.256,P= 0.061 and r= -0.121,P= 0.385),and serum HBsAg level was not correlated significantly with both AR mRNA and ERαmRNA level in liver tissue (r= 0.130,P= 0.348 and r= 0.165,P= 0.234);HBcAg expression level in liver tissue was not correlated significantly with both AR mRNA and ERαmRNA level in liver tissue (rs= -0.053,P= 0.701 and rs= -0.203,P= 0.137),and HBsAg expression level in liver tissue was not correlated significantly with both AR mRNA and ERαmRNA level in liver tissue (rs= 0.159,P=0.247 and rs= 0.192,P= 0.160).The outcomes of multivariate analyses of variance and ordinal logistic regression analyses showed that AR mRNA level in liver tissue was the independent factor influencing HBsAg level in serum and HBcAg expression level in liver tissue. Conclusion AR level in liver tissue is an independent factor influencing antigen expression of hepatitis B virus in patients with CHB.