甘肃农业科技
甘肅農業科技
감숙농업과기
GANSU AGRICULTURAL SCIENCE AND TECHNOLOGY
2014年
10期
16-18,19
,共4页
番茄%黄化曲叶病毒病%侵染性克隆%抗性鉴定
番茄%黃化麯葉病毒病%侵染性剋隆%抗性鑒定
번가%황화곡협병독병%침염성극륭%항성감정
Yellow leaf curl virus%Infectious clones%Resistance evaluation
采用 YEP1和 YEP2培养基培养番茄黄化曲叶病毒侵染性克隆 pBinPLUS-1.7A+2β，并用固体菌落、液体菌菌液浓度 OD600≈0.6、液体菌菌液浓度 OD600≈1.0对感病番茄品种 MM、中蔬4号、98-B1在4～6叶苗期进行接种处理。结果显示，2种液体培养基的液体菌菌液浓度为 OD600≈0.6时接种处理发病率较高，并初步确立了无 TY 发病地区侵染性克隆接种鉴定方法。
採用 YEP1和 YEP2培養基培養番茄黃化麯葉病毒侵染性剋隆 pBinPLUS-1.7A+2β，併用固體菌落、液體菌菌液濃度 OD600≈0.6、液體菌菌液濃度 OD600≈1.0對感病番茄品種 MM、中蔬4號、98-B1在4～6葉苗期進行接種處理。結果顯示，2種液體培養基的液體菌菌液濃度為 OD600≈0.6時接種處理髮病率較高，併初步確立瞭無 TY 髮病地區侵染性剋隆接種鑒定方法。
채용 YEP1화 YEP2배양기배양번가황화곡협병독침염성극륭 pBinPLUS-1.7A+2β，병용고체균락、액체균균액농도 OD600≈0.6、액체균균액농도 OD600≈1.0대감병번가품충 MM、중소4호、98-B1재4～6협묘기진행접충처리。결과현시，2충액체배양기적액체균균액농도위 OD600≈0.6시접충처리발병솔교고，병초보학립료무 TY 발병지구침염성극륭접충감정방법。
The pBinPLUS-1.7A+2β was nourished in two species medium, and set up three different treatments respectively, such as colonies, the two concentration of agrobacterium is about OD600≈0.6 or OD600≈1.0. The 4~6 leaves seedlings were inoculated in susceptible tomato varieties MM, Zhongshu 4, and 98-B1 . The results shows that it was higher TY incidence when the two concentration of agrobacterium is about OD600≈0.6 . The techniques of pBinPLUS-1.7A+2β inoculation identification is established preliminarily in the areas, where the TY has not happened.
