CAJ | 학술논문

分别采用试剂盒和CTAB法提取小麦干种子基因组DNA， DNA样品经紫外光谱吸收、琼脂糖凝胶电泳、PCR扩增检测。结果表明，试剂盒提取DNA操作过程快速简便、成本较低， DNA产率和纯度优于CTAB法。通过多重PCR技术分别以小麦LMW-GS亚基Glu-B3位点和黑麦碱secalin-p基因的特异引物进行扩增，均能够扩增出清晰的目标条带，提取的基因组DNA能够满足分子检测的实验要求。
분별채용시제합화CTAB법제취소맥간충자기인조DNA， DNA양품경자외광보흡수、경지당응효전영、PCR확증검측。결과표명，시제합제취DNA조작과정쾌속간편、성본교저， DNA산솔화순도우우CTAB법。통과다중PCR기술분별이소맥LMW-GS아기Glu-B3위점화흑맥감secalin-p기인적특이인물진행확증，균능구확증출청석적목표조대，제취적기인조DNA능구만족분자검측적실험요구。
Two rapid DNA isolation methods from dry seeds of wheat was established by DNA extraction kit and CTAB in this study. The DNA samples were detected in terms of the UV spectrophotometer analysis, agarose gel electrophoresis, PCR amplification respectively. The results indicats that the method with DNA Extraction Kits was simple, rapid and low costs, the extraction rate and purity of DNA was better than that of the CTAB. Using the DNA samples as template, the Glu-B3 locus of wheat LMW-GS andω-secalin gene of triticale could be amplified with distinct and specific target bands by multiplex PCR. The genomic DNA extracted from dry seeds of wheat can meet the needs of molecular tests.