CAJ | 학술논문

本文以灭活非洲猪瘟病毒为包被抗原，分析了包被浓度、酶标二抗、显色底物对ELISA法检测非洲猪瘟抗体的影响，以期获得最适反应条件。以2个阳性血清和一个阴性血清为待检样品，用不同包被浓度、2种酶标二抗和2种显色底物进行ELISA反应，记录吸光值，分析阴阳性样品吸光值差异，优化阴性样品背景值，计算P/N值。结果表明，包被浓度为15μg/mL、二抗为HRP标记蛋白A、显色底物为OPD时，两个阳性样品P/N值分别为4.920和7.259，为本方法的最适反应条件。
본문이멸활비주저온병독위포피항원，분석료포피농도、매표이항、현색저물대ELISA법검측비주저온항체적영향，이기획득최괄반응조건。이2개양성혈청화일개음성혈청위대검양품，용불동포피농도、2충매표이항화2충현색저물진행ELISA반응，기록흡광치，분석음양성양품흡광치차이，우화음성양품배경치，계산P/N치。결과표명，포피농도위15μg/mL、이항위HRP표기단백A、현색저물위OPD시，량개양성양품P/N치분별위4.920화7.259，위본방법적최괄반응조건。
In the study,the effects of antigen coating concentration,enzyme labeled antibody and chromogenic sub-strate in an ELISA with inactivated African swine fever virus as coating antigen were analyzed for detection of ASFV antibody. In order to obtain the optimum reaction conditions,different coating antigen concentrations,2 type enzyme-labeled antibody and 2 type chromogenic substrates were used in the ELISA to detect 2 positive serum samples and 1 negative serum sample. The result showed that the optimum reaction conditions were:the antigen coating concentration was 15μg/mL;Protein A-HRP was used as labeled antibody,and OPD as chromogenic substrate and the P/N value of 2 positive serum samples were 4.920 and 7.259.