CAJ | 학술논문

[目的]了解广东从化无规定马属动物疫病区野鸟和蚊子的种类、数量和分布等情况及其西尼罗病毒感染的情况。[方法]对鸟类在沿途设网抓捕取样；蚊虫采用紫外灯诱捕和网捕进行样品采集，随后利用荧光定量RT-PCR方法对西尼罗病毒进行检测。[结果]2012-2013年该地区共捕获鸟类110种381只，隶属于12目37科76属；共捕获蚊虫5种，共计3048只，其中淡色库蚊占50.66%、三带喙库蚊占32.12%、褐尾库蚊占12.99%、中华按蚊占3.61%和白纹伊蚊0.62%；对381份鸟泄殖腔棉拭子和3048份蚊子样品进行了西尼罗病毒的检测，结果均为阴性。[结论]虽然本次调查未发现鸟类及蚊子感染西尼罗病毒，但本地区存在西尼罗病毒引入以及在本地区传播的条件，存在西尼罗病毒病发生风险。因此，为继续维持广东从化无规定马属动物疫病区的无疫状态，有必要对媒介鸟类及蚊虫开展长期的调查和监测。
[목적]료해엄동종화무규정마속동물역병구야조화문자적충류、수량화분포등정황급기서니라병독감염적정황。[방법]대조류재연도설망조포취양；문충채용자외등유포화망포진행양품채집，수후이용형광정량RT-PCR방법대서니라병독진행검측。[결과]2012-2013년해지구공포획조류110충381지，대속우12목37과76속；공포획문충5충，공계3048지，기중담색고문점50.66%、삼대훼고문점32.12%、갈미고문점12.99%、중화안문점3.61%화백문이문0.62%；대381빈조설식강면식자화3048빈문자양품진행료서니라병독적검측，결과균위음성。[결론]수연본차조사미발현조류급문자감염서니라병독，단본지구존재서니라병독인입이급재본지구전파적조건，존재서니라병독병발생풍험。인차，위계속유지엄동종화무규정마속동물역병구적무역상태，유필요대매개조류급문충개전장기적조사화감측。
Objective To investigate the species,number and distribution of wild birds and mosquitoes and West Nile virus(WNV)the specific equine disease-free zone of Conghua,Guangdong.Method The birds were captured on the migration routes with nets,and mosquitoes by Ultraviolet light trapping and nets.The samples were detected with real-time RT-PCR assay for West Nile virus.Result All the 381 birds of 110 species and 3048 mosquitoes of 5 species collected were negative for West Nile virus.Conclusion No WNV-positive birds and mosquitoes were detected in the survey.There were amount of potential vectors of WNV found in the zone.To maintain the disease-free status of the specifi c equine disease-free zone of Conghua,further work was needed to monitor the bird and mosquito activities for long time.