CAJ | 학술논문

为了解黑龙江省B镇肉牛的布鲁氏菌病流行现状，从而评估该病在当地牛群中的流行状况及人感染布鲁氏菌的风险，本研究以估计个体及群流行率为目的，按照预期流行率0.5%，可接受误差0.3%，置信水平95%对B镇531个肉牛养殖场户养殖的3535头肉牛进行随机抽样，获得血清样本1334份，用虎红平板凝集试验和试管凝集试验两种方法检测均为阳性的牛即为布病患病牛，同时对养殖户就养殖模式、动物来源、更新方式、既往病史、养殖规模等方面进行问卷调查。血清检测结果显示，虎红平板凝集5份阳性，阳性率为0.37%，对5份阳性血清进行试管凝集试验复检，布鲁氏菌抗体全部为阴性。同时回收问卷531份，有效问卷389份，合格率73.3%。根据本次检测结果，B镇肉牛布病个体流行率接近于零，牛群中没有发现利于布鲁氏菌感染的风险因素。
위료해흑룡강성B진육우적포로씨균병류행현상，종이평고해병재당지우군중적류행상황급인감염포로씨균적풍험，본연구이고계개체급군류행솔위목적，안조예기류행솔0.5%，가접수오차0.3%，치신수평95%대B진531개육우양식장호양식적3535두육우진행수궤추양，획득혈청양본1334빈，용호홍평판응집시험화시관응집시험량충방법검측균위양성적우즉위포병환병우，동시대양식호취양식모식、동물래원、경신방식、기왕병사、양식규모등방면진행문권조사。혈청검측결과현시，호홍평판응집5빈양성，양성솔위0.37%，대5빈양성혈청진행시관응집시험복검，포로씨균항체전부위음성。동시회수문권531빈，유효문권389빈，합격솔73.3%。근거본차검측결과，B진육우포병개체류행솔접근우령，우군중몰유발현리우포로씨균감염적풍험인소。
Epidemiological research methods were adopted to estimate prevalence and identify risk factors of brucellosis in the beef cattle in B town of Heilongjiang province. Sample size was determined for the purpose of estimating prevalence with estimated prevalence 0.5%,acceptable error 0.3% and confi dence level 95% as parameters in the calculation. A total of 1334 serum samples were collected from 531 beef cattle farms and tested using RBPT and SAT. A positive sample should be confi rmed by both of the two tests. In addition,questionnaires were fi lled out during the investigation,including farm type information,animal source,management mode,disease history,farm size, etc. The serological tests showed that five samples were positive for antibodies toBrucella in the RBPT,but they were all negative in the SAT test. 531 questionnaires were collected with 389 as valid,yielding a valid rate of 73.3%. Considering the specifi city of the test,the true cattle prevalence of brucellosis was approximately nil and no risk factor was identifi ed in the study.