海南师范大学学报（自然科学版）
海南師範大學學報（自然科學版）
해남사범대학학보（자연과학판）
JOURNAL OF HAINAN NORMAL UNIVERSITY(NATURAL SCIENCE)
2014年
3期
277-279,323
,共4页
微羟自由基%日落黄%H2O2-Fe(II)%EDTA-Fe(II)
微羥自由基%日落黃%H2O2-Fe(II)%EDTA-Fe(II)
미간자유기%일락황%H2O2-Fe(II)%EDTA-Fe(II)
Hydroxyl radical%Sunset yellow%H2O2-Fe(II)%EDTA-Fe(II)
利用Fenton反应中加色素日落黄（FCF），产生的羟自由基（·OH）氧化日落黄发生减色效应.加入抑制·OH的还原剂，测定日落黄吸光度的变化间接测定羟自由基的产生量.实验对抗坏血酸，盐酸羟胺在不同溶剂中的抗羟基自由基氧化性能进行了比较.结果表明：抗坏血酸、盐酸羟胺50%乙醇溶液的抗氧化能力较抗坏血酸、盐酸羟胺水溶液的抗氧化能力强.4种体系中的回收率分别为：抗坏血酸107.2%、盐酸羟胺50%乙醇溶液104.2%，抗坏血酸90.2%、盐酸羟胺水溶液96.0%.
利用Fenton反應中加色素日落黃（FCF），產生的羥自由基（·OH）氧化日落黃髮生減色效應.加入抑製·OH的還原劑，測定日落黃吸光度的變化間接測定羥自由基的產生量.實驗對抗壞血痠，鹽痠羥胺在不同溶劑中的抗羥基自由基氧化性能進行瞭比較.結果錶明：抗壞血痠、鹽痠羥胺50%乙醇溶液的抗氧化能力較抗壞血痠、鹽痠羥胺水溶液的抗氧化能力彊.4種體繫中的迴收率分彆為：抗壞血痠107.2%、鹽痠羥胺50%乙醇溶液104.2%，抗壞血痠90.2%、鹽痠羥胺水溶液96.0%.
이용Fenton반응중가색소일락황（FCF），산생적간자유기（·OH）양화일락황발생감색효응.가입억제·OH적환원제，측정일락황흡광도적변화간접측정간자유기적산생량.실험대항배혈산，염산간알재불동용제중적항간기자유기양화성능진행료비교.결과표명：항배혈산、염산간알50%을순용액적항양화능력교항배혈산、염산간알수용액적항양화능력강.4충체계중적회수솔분별위：항배혈산107.2%、염산간알50%을순용액104.2%，항배혈산90.2%、염산간알수용액96.0%.
Using the mechanism that hydroxyl free radicals produced by Fenton reaction can make the sunset yellow fade, the discharge of hydroxyl free radicals was measured indirectly. The resistance of performance of ascorbic acid and hydroxyl-amine hydrochloride in different soluion to the hydroxyl free radical oxidation were compared. Results showed that the anti-oxidant capacity of ascorbic acid and hydroxylamine hydrochloride in 50%ethanol solution is better than ascorbic acid and hydroxylamine hydrochloride in aqueous solution. The recovery rates of ascorbic acid and hydroxylamine hydrochloride in 50%ethanol solution are 107.2%, 104.2%respectively. The recovery rates of ascorbic acid and hydroxylamine hydrochlo-ride in aqueous solution are 90.2%, 96.0%respectively.
