中国神经免疫学和神经病学杂志
中國神經免疫學和神經病學雜誌
중국신경면역학화신경병학잡지
CHINESE JOURNAL OF NEUROIMMUNOLOGY AND
2014年
3期
196-198,202
,共4页
郝娟%于顺%陈彪%李尧华
郝娟%于順%陳彪%李堯華
학연%우순%진표%리요화
帕金森病%T 淋巴细胞亚群%T 淋巴细胞 ,辅助诱导%纯真 T 细胞%记忆 T 细胞%T 淋巴细胞 ,调节性
帕金森病%T 淋巴細胞亞群%T 淋巴細胞 ,輔助誘導%純真 T 細胞%記憶 T 細胞%T 淋巴細胞 ,調節性
파금삼병%T 림파세포아군%T 림파세포 ,보조유도%순진 T 세포%기억 T 세포%T 림파세포 ,조절성
Parkinson’ s disease%T lymphocytes subsets%T lymphocytes,helper-inducer%naive T cell%memory T cell%T lymphocytes,regulatory
目的：研究帕金森病（PD）患者外周血淋巴细胞亚群的变化，并探讨其在疾病进程中的意义。方法选取典型的原发性 PD 患者34例及健康对照35名，采用流式细胞术检测外周血淋巴细胞中纯真（naive）、记忆（memory）及调节性（regulatory）T 细胞亚群百分比，比较两组间各 T 细胞亚群的差异。结果与对照组比较，PD 患者外周血 T 淋巴细胞中杀伤性纯真 T 细胞亚群（CD8＋ CD45RA ＋ CD45RO －）百分比明显增高（P ＜0.01），杀伤性记忆 T 细胞亚群（CD8＋ CD45RA － CD45RO ＋）和调节性 T 细胞亚群（CD4＋ CD25＋ FoxP3＋）百分比均明显降低（P ＜0.01），而辅助性纯真 T 细胞亚群（CD4＋ CD45RA ＋ CD45RO －）和辅助性记忆 T 细胞亚群（CD4＋ CD45RA － CD45RO ＋）与健康者比较无统计学差异。结论 PD 患者外周血淋巴细胞中杀伤性纯真 T 细胞亚群、杀伤性记忆 T 细胞亚群及调节性 T 细胞亚群百分比与健康者间存在统计学差异，细胞免疫失衡可能参与了 PD 的病理进程。
目的：研究帕金森病（PD）患者外週血淋巴細胞亞群的變化，併探討其在疾病進程中的意義。方法選取典型的原髮性 PD 患者34例及健康對照35名，採用流式細胞術檢測外週血淋巴細胞中純真（naive）、記憶（memory）及調節性（regulatory）T 細胞亞群百分比，比較兩組間各 T 細胞亞群的差異。結果與對照組比較，PD 患者外週血 T 淋巴細胞中殺傷性純真 T 細胞亞群（CD8＋ CD45RA ＋ CD45RO －）百分比明顯增高（P ＜0.01），殺傷性記憶 T 細胞亞群（CD8＋ CD45RA － CD45RO ＋）和調節性 T 細胞亞群（CD4＋ CD25＋ FoxP3＋）百分比均明顯降低（P ＜0.01），而輔助性純真 T 細胞亞群（CD4＋ CD45RA ＋ CD45RO －）和輔助性記憶 T 細胞亞群（CD4＋ CD45RA － CD45RO ＋）與健康者比較無統計學差異。結論 PD 患者外週血淋巴細胞中殺傷性純真 T 細胞亞群、殺傷性記憶 T 細胞亞群及調節性 T 細胞亞群百分比與健康者間存在統計學差異，細胞免疫失衡可能參與瞭 PD 的病理進程。
목적：연구파금삼병（PD）환자외주혈림파세포아군적변화，병탐토기재질병진정중적의의。방법선취전형적원발성 PD 환자34례급건강대조35명，채용류식세포술검측외주혈림파세포중순진（naive）、기억（memory）급조절성（regulatory）T 세포아군백분비，비교량조간각 T 세포아군적차이。결과여대조조비교，PD 환자외주혈 T 림파세포중살상성순진 T 세포아군（CD8＋ CD45RA ＋ CD45RO －）백분비명현증고（P ＜0.01），살상성기억 T 세포아군（CD8＋ CD45RA － CD45RO ＋）화조절성 T 세포아군（CD4＋ CD25＋ FoxP3＋）백분비균명현강저（P ＜0.01），이보조성순진 T 세포아군（CD4＋ CD45RA ＋ CD45RO －）화보조성기억 T 세포아군（CD4＋ CD45RA － CD45RO ＋）여건강자비교무통계학차이。결론 PD 환자외주혈림파세포중살상성순진 T 세포아군、살상성기억 T 세포아군급조절성 T 세포아군백분비여건강자간존재통계학차이，세포면역실형가능삼여료 PD 적병리진정。
Objective To investigate the percentage and significance of naive ,memory ,and regulatory T lymphocyte subgroups in the peripheral blood of patients with Parkinson’ s disease (PD) .Methods Thirty‐four PD patients were enrolled .The percentages of naive T cells (CD4 + CD45RA + CD45RO - ,CD8 + CD45RA +CD45RO - ) ,memory T cells (CD4 + CD45RA - CD45RO + ,CD8 + CD45RA - CD45RO + ) and regulatory T cells (CD4 + CD25 + Foxp3 + ) were detected and analyzed by flow cytometry and compared with 35 normal controls . Results The percentage of CD8 + CD45RA + CD45RO - cells in the PD group was higher (P < 0.01) ,while the percentages of CD8 + CD45RA - CD45RO + and CD4 + CD25 + FoxP3 + cells in the PD group were lower than those of the control group ( P < 0.01 ) . There was no significant difference of the percentages of CD4 + CD45RA +CD45RO - and CD4 + CD45RA - CD45RO + cells between the PD group and the control group .Conclusions The alterations of the percentages of CD8 + CD45RA - CD45RO + , CD8 + CD45RA + CD45RO - and CD4 + CD25 +FoxP3 + cells in the PD group lead to the cell immune disturbance ,which may play roles in the pathological progress of the PD .
