CAJ | 학술논문

目的：观察自噬及自噬相关蛋白 Beclin1及微管相关蛋白1轻链3（microtubule‐associated protein 1 light chain 3，MAPlLC3，简称 LC3）在帕金森病（Parkinson disease ，PD）模型大鼠黑质纹状体中的表达及变化，探讨自噬在 PD 发病中的作用。方法将90只 SD 大鼠随机分为正常组、假手术组、PD 模型组（模型组），每组又分为模型制备后4 d 、8 d 两个亚组，每个亚组各15只。采用颈背部皮下注射鱼藤酮制作 PD 模型，透射电镜观察自噬体的形成，免疫组化法和 Western blotting 法检测黑质纹状体自噬相关蛋白 Beclin1和 LC3的表达水平。结果（1）电子显微镜下 PD 模型组黑质纹状体中出现自噬体，呈圆形，大多位于细胞核旁，溶酶体数量增多。8 d 时，自噬体、溶酶体数量显著增多。（2）与正常组比较，假手术组黑质纹状体中 Beclin1和 LC3表达略增多，但差异无统计学意义（均 P＞0.05）；与假手术组比较，模型组黑质纹状体 Beclin1和 LC3的表达明显增多（均 P＜0.01），8 d 高于4 d ，差异有统计学意义（均 P＜0.05）。结论 PD 模型大鼠黑质纹状体中自噬活性被激活，自噬可能参与了 PD 的发病过程。
목적：관찰자서급자서상관단백 Beclin1급미관상관단백1경련3（microtubule‐associated protein 1 light chain 3，MAPlLC3，간칭 LC3）재파금삼병（Parkinson disease ，PD）모형대서흑질문상체중적표체급변화，탐토자서재 PD 발병중적작용。방법장90지 SD 대서수궤분위정상조、가수술조、PD 모형조（모형조），매조우분위모형제비후4 d 、8 d 량개아조，매개아조각15지。채용경배부피하주사어등동제작 PD 모형，투사전경관찰자서체적형성，면역조화법화 Western blotting 법검측흑질문상체자서상관단백 Beclin1화 LC3적표체수평。결과（1）전자현미경하 PD 모형조흑질문상체중출현자서체，정원형，대다위우세포핵방，용매체수량증다。8 d 시，자서체、용매체수량현저증다。（2）여정상조비교，가수술조흑질문상체중 Beclin1화 LC3표체략증다，단차이무통계학의의（균 P＞0.05）；여가수술조비교，모형조흑질문상체 Beclin1화 LC3적표체명현증다（균 P＜0.01），8 d 고우4 d ，차이유통계학의의（균 P＜0.05）。결론 PD 모형대서흑질문상체중자서활성피격활，자서가능삼여료 PD 적발병과정。
Objective To observe the expression and variation of autophagy ,Beclin1 and microtubule‐associated protein 1 light chain 3 (LC3) in substantia nigra and striatum of rat models of Parkinson’ s disease (PD) ,and to explore the role of autophagy in the pathogenesis of PD .Methods Ninety rats were randomly divided into the normal control group ,the sham operation group ,and the model group .Each group was divided into 4 days and 8 days subgroups after successful model preparation (n= 15) .PD rat model was established by injecting rotenone subcutaneously . The autophagy was observed by transmission electron microscope . The expression of Beclin1 and LC3 in substantia nigra and striatum was detected by immunohistochemistry and western blotting .Results (1) Under the electron microscope autophagosomes appeared in substantia nigra and striatum of the model group .They were round ,mostly located beside the nucleus ,and the number of lysosomes increased .Both of them significantly increased in 8 d .(2) The expression of Beclin1 and LC3 in substantia nigra and striatum slightly increased in the sham operation group compared with that in the normal group ,but the difference was not statistically significant (all P> 0.05) .The expression of Beclin1 and LC3 in substantia nigra and striatum increased in the model group compared with that in the sham operation group (all P< 0.01) , and there were more expression of Beclin1 and LC3 at 8 d compared with those at 4 d ,and it has significant difference (all P< 0.05) .Conclusions Autophagy is activated in substantia nigra and striatum of rat models of PD and it may be involved in the pathogenesis of PD .