中国神经免疫学和神经病学杂志
中國神經免疫學和神經病學雜誌
중국신경면역학화신경병학잡지
CHINESE JOURNAL OF NEUROIMMUNOLOGY AND
2014年
3期
153-156
,共4页
朱德生%谢冲%李世旭%管阳太
硃德生%謝遲%李世旭%管暘太
주덕생%사충%리세욱%관양태
脑脊髓炎 ,自身免疫性 ,实验性%脱髓鞘疾病%S-100B 蛋白%S100 蛋白质类
腦脊髓炎 ,自身免疫性 ,實驗性%脫髓鞘疾病%S-100B 蛋白%S100 蛋白質類
뇌척수염 ,자신면역성 ,실험성%탈수초질병%S-100B 단백%S100 단백질류
encephalomyelitis,autoimmune,experimental%demyelinating disease%S-100B protein
目的：探讨实验性自身免疫性脑脊髓炎（experimental autoimmune encephalomyelitis ，EAE ）大鼠血清 S‐100B 蛋白浓度的动态变化及其在中枢神经脱髓鞘疾病中的临床意义。方法将 Wistar 大鼠随机分为EAE 模型组与正常对照组。 EAE 模型组经皮下注射抗原乳剂与百日咳杆菌免疫诱导为 EAE 大鼠；正常对照组单纯皮下注射生理盐水。按 Benson 评分标准评估大鼠临床神经功能；采用双抗体夹心酶标免疫分析法测定大鼠血清 S‐100B 值。比较两组大鼠临床神经功能评分、血清 S‐100B 水平。结果与对照组比较，实验组临床神经功能评分在免疫后第12、16、20、24、28天时增高（P＜0.01）；对照组与实验组之间血清 S‐100B 蛋白值总体比较有统计学差异（F＝1320.291，P＜0.01）；与对照组比较，实验组血清 S‐100B 蛋白值在免疫后第4～28天时增高（P＜0.01）；临床神经功能评分与血清 S‐100B 值呈正相关（r ＝0.959，P ＜0.01）。结论血清 S‐100B 蛋白可早期反映中枢神经脱髓鞘病变，可作为监测中枢神经脱髓鞘病变的生化指标，有助于早期诊断、病情监测及预后评估。
目的：探討實驗性自身免疫性腦脊髓炎（experimental autoimmune encephalomyelitis ，EAE ）大鼠血清 S‐100B 蛋白濃度的動態變化及其在中樞神經脫髓鞘疾病中的臨床意義。方法將 Wistar 大鼠隨機分為EAE 模型組與正常對照組。 EAE 模型組經皮下註射抗原乳劑與百日咳桿菌免疫誘導為 EAE 大鼠；正常對照組單純皮下註射生理鹽水。按 Benson 評分標準評估大鼠臨床神經功能；採用雙抗體夾心酶標免疫分析法測定大鼠血清 S‐100B 值。比較兩組大鼠臨床神經功能評分、血清 S‐100B 水平。結果與對照組比較，實驗組臨床神經功能評分在免疫後第12、16、20、24、28天時增高（P＜0.01）；對照組與實驗組之間血清 S‐100B 蛋白值總體比較有統計學差異（F＝1320.291，P＜0.01）；與對照組比較，實驗組血清 S‐100B 蛋白值在免疫後第4～28天時增高（P＜0.01）；臨床神經功能評分與血清 S‐100B 值呈正相關（r ＝0.959，P ＜0.01）。結論血清 S‐100B 蛋白可早期反映中樞神經脫髓鞘病變，可作為鑑測中樞神經脫髓鞘病變的生化指標，有助于早期診斷、病情鑑測及預後評估。
목적：탐토실험성자신면역성뇌척수염（experimental autoimmune encephalomyelitis ，EAE ）대서혈청 S‐100B 단백농도적동태변화급기재중추신경탈수초질병중적림상의의。방법장 Wistar 대서수궤분위EAE 모형조여정상대조조。 EAE 모형조경피하주사항원유제여백일해간균면역유도위 EAE 대서；정상대조조단순피하주사생리염수。안 Benson 평분표준평고대서림상신경공능；채용쌍항체협심매표면역분석법측정대서혈청 S‐100B 치。비교량조대서림상신경공능평분、혈청 S‐100B 수평。결과여대조조비교，실험조림상신경공능평분재면역후제12、16、20、24、28천시증고（P＜0.01）；대조조여실험조지간혈청 S‐100B 단백치총체비교유통계학차이（F＝1320.291，P＜0.01）；여대조조비교，실험조혈청 S‐100B 단백치재면역후제4～28천시증고（P＜0.01）；림상신경공능평분여혈청 S‐100B 치정정상관（r ＝0.959，P ＜0.01）。결론혈청 S‐100B 단백가조기반영중추신경탈수초병변，가작위감측중추신경탈수초병변적생화지표，유조우조기진단、병정감측급예후평고。
Objective To explore the dynamic changes in serum S‐100B protein of rats with experimental autoimmune encephalomyelitis ( EAE ) and its clinical significance in the central nervous demyelinating disease .Methods Wistar rats were randomly divided into the EAE model group and the normal control group .Rats in the EAE model group were injected with pertussis antigen emulsion subcutaneously and induced as EAE rats . Rats in the normal control group were injected with saline subcutaneously . Clinical neurological function was assessed by Benson scoring criteria , the levels of serum S‐100B protein in the experimental group and the normal control group were detected respectively by the method “double‐antibody sandwich enzyme immunoassay” . Clinical neurology score and serum S‐100B value of the two groups were analyzed .Results Compared with the control group ,clinical scores of the experimental group increased on the days of 12 ,16 ,20 ,24 and 28 ( P < 0.01) . The difference of serum S‐100B protein values was statistically significant between the two groups (F= 1320.291 ,P< 0.01) .Compared with the control group ,Serum S‐100B protein of the experimental group increased on the days of 4 , 8 , 12 , 16 , 20 , 24 ,and 28 (P < 0.01) .There was positive correlation between clinical neurological function and serum S‐100B values (r = 0.959 , P < 0.01) . Conclusions Serum S‐100B protein can reflect the demyelinating changes in the central nervous system .It can be a biochemical indicator to monitor the demyelinating process of the central nervous system ,and is helpful for early diagnosis ,disease surveillance ,and judging the prognosis of demyelinating diseases .
