中国实验动物学报
中國實驗動物學報
중국실험동물학보
ACTA LABORATORIUM ANIMALIS SCIENTIA SINICA
2014年
5期
75-78
,共4页
李粮辉%陈文华%郑宏
李糧輝%陳文華%鄭宏
리량휘%진문화%정굉
缺血再灌注%心肌梗死面积%2 ,3 ,5-氯化三苯基四氮唑%染色%大鼠
缺血再灌註%心肌梗死麵積%2 ,3 ,5-氯化三苯基四氮唑%染色%大鼠
결혈재관주%심기경사면적%2 ,3 ,5-록화삼분기사담서%염색%대서
Ischemia/reperfusion%Myocardium,infarct size%2,3,5-triphenyltetrazolium chloride%Staining%Rat
目的：比较应用不同2，3，5-氯化三苯基四氮唑（TTC）染色方法对大鼠心肌缺血再灌注损伤后梗死面积的检测效果。方法将20只SD大鼠（雄性，8周龄，体重250～300 g）按随机数字表法分为两组，每组10只。A组：传统TTC染色法组；B组：改进后的TTC染色法组，分别进行大鼠心肌染色，随后计算心肌梗死面积及测定血清cTnI浓度水平。结果 A组和B组均能较好地标记梗死心肌；A组和B组心肌梗死面积百分比无统计学差异（48.69％±5.37％vs．47.41％±3.28％，P＞0.05）；A组和B组血清cTnI浓度水平无统计学差异（4.51±0.88 ng/mL vs．4.70±0.71 ng/mL，P＞0.05）；但B组心肌切片染色色泽对比度及心肌非梗死区与梗死区区分度均高于A组。结论改进后的心肌TTC染色法采用在体染色，不仅操作简便，节省了实验时间和经费，而且提高了染色效果，能更准确地反映心肌缺血再灌注损伤的程度。因此改进后的心肌TTC染色法是一种经济、简便、快捷、高效的染色方法。
目的：比較應用不同2，3，5-氯化三苯基四氮唑（TTC）染色方法對大鼠心肌缺血再灌註損傷後梗死麵積的檢測效果。方法將20隻SD大鼠（雄性，8週齡，體重250～300 g）按隨機數字錶法分為兩組，每組10隻。A組：傳統TTC染色法組；B組：改進後的TTC染色法組，分彆進行大鼠心肌染色，隨後計算心肌梗死麵積及測定血清cTnI濃度水平。結果 A組和B組均能較好地標記梗死心肌；A組和B組心肌梗死麵積百分比無統計學差異（48.69％±5.37％vs．47.41％±3.28％，P＞0.05）；A組和B組血清cTnI濃度水平無統計學差異（4.51±0.88 ng/mL vs．4.70±0.71 ng/mL，P＞0.05）；但B組心肌切片染色色澤對比度及心肌非梗死區與梗死區區分度均高于A組。結論改進後的心肌TTC染色法採用在體染色，不僅操作簡便，節省瞭實驗時間和經費，而且提高瞭染色效果，能更準確地反映心肌缺血再灌註損傷的程度。因此改進後的心肌TTC染色法是一種經濟、簡便、快捷、高效的染色方法。
목적：비교응용불동2，3，5-록화삼분기사담서（TTC）염색방법대대서심기결혈재관주손상후경사면적적검측효과。방법장20지SD대서（웅성，8주령，체중250～300 g）안수궤수자표법분위량조，매조10지。A조：전통TTC염색법조；B조：개진후적TTC염색법조，분별진행대서심기염색，수후계산심기경사면적급측정혈청cTnI농도수평。결과 A조화B조균능교호지표기경사심기；A조화B조심기경사면적백분비무통계학차이（48.69％±5.37％vs．47.41％±3.28％，P＞0.05）；A조화B조혈청cTnI농도수평무통계학차이（4.51±0.88 ng/mL vs．4.70±0.71 ng/mL，P＞0.05）；단B조심기절편염색색택대비도급심기비경사구여경사구구분도균고우A조。결론개진후적심기TTC염색법채용재체염색，불부조작간편，절성료실험시간화경비，이차제고료염색효과，능경준학지반영심기결혈재관주손상적정도。인차개진후적심기TTC염색법시일충경제、간편、쾌첩、고효적염색방법。
Objective To evaluate an improved modification of TTC staining method for measuring myocardial in-farct size after ischemia-reperfusion in rats.Methods Twenty healthy SPF male 8-week-old SD rats were randomly divided into two groups:Group A with conventional TTC staining, and group B with the modified TTC staining method for measuring myocardial ischemia-reperfusion injury.The infarct size was caculated and the serum cTnI levels were determined.Results The infarcted myocardium was well detected in both groups A and B.There were no significant differences in the myocardial infarct sizes measured in the groups A and B (48.69 ±5.37 %vs.47.41 ±3.28%, P>0.05).There were no significant differences in the serum cTnI levels assayed in the groups A and B (4.51 ±0.88 ng/mL vs.4.70 ±0.71 ng/mL, P>0.05). But compared with the group A, the color contrast of stained myocardial slice and the distinguishing infarction area and non-infarction area were much clearer in the group B.Conclusions Our modified TTC staining technique using in vivo staining is an economic, convenient, fast and efficient method, being easy to control, time-saving and inexpensive, and enhances the staining effect in evaluating the size of myocardial ischemia/reperfusion injury more accurately.
